A simultaneous analysis method for Caihu-Dayuan-Yin using reversed-phase high-performance liquid chromatography coupled with pulsed amperometric detection

• Published in: Archives of pharmacal research Vol. 36; no. 5; pp. 579 - 585
• Main Authors: Kwon, Ha-Jeong, Kim, Ji-Hwan, Park, Yong-Duk, Hong, Seon-Pyo
• Format: Journal Article
• Language: English
• Published: Heidelberg Pharmaceutical Society of Korea 01.05.2013; 대한약학회
• Subjects: Calibration; Chromatography, High Pressure Liquid - methods; Chromatography, Reverse-Phase - methods; detection limit; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - isolation & purification; Electrochemical Techniques - methods; herbal medicines; Limit of Detection; Medicine; Medicine, Chinese Traditional; Molecular Structure; Pharmacology/Toxicology; Pharmacy; Plants, Medicinal; Reproducibility of Results; Research Article; reversed-phase high performance liquid chromatography; 약학; Pulsed amperometric detection; Sensitivity; Reversed-phased high performance liquid chromatography; Caihu-Dayuan-Yin
• ISSN: 0253-6269; 1976-3786; 1976-3786
• DOI: 10.1007/s12272-013-0040-7

A simultaneous analysis method was developed for Caihu-Dayuan-Yin decoction containing Bupleuri Radix, Glycyrrhizae Radix, and Ponciri fructus by reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD). The analytes were separated in a RP column with 0.01 % phosphoric acid–acetonitrile gradient elution. The limits of detection ( S / N  = 3) and of quantification ( S / N  = 10) ranged 0.1–3.0, and 0.3–10 ng, respectively. The intra- and interday precisions (RSDs) were ≤15.15 % and the average recoveries were 94.6–98.3 %. Our RP-HPLC–PAD method showed 1.7–100 times higher sensitivity than the RP-HPLC–UV method.