miR-7641 modulates the expression of CXCL1 during endothelial differentiation derived from human embryonic stem cells

• Published in: Archives of pharmacal research Vol. 36; no. 3; pp. 353 - 358
• Main Authors: Yoo, Jung Ki, Jung, Ho Yong, Kim, Chang-Hyun, Son, Woo Sung, Kim, Jin Kyeoung
• Format: Journal Article
• Language: English
• Published: Heidelberg Pharmaceutical Society of Korea 01.03.2013; 대한약학회
• Subjects: angiogenesis; Base Sequence; biogenesis; Cell Differentiation - physiology; Cells, Cultured; chemokine CXCL1; Chemokine CXCL1 - biosynthesis; Chemokine CXCL1 - genetics; embryonic stem cells; Embryonic Stem Cells - physiology; endothelial cells; G-protein coupled receptors; gene expression; Gene Expression Regulation; genes; Humans; luciferase; Medicine; microRNA; MicroRNAs - genetics; MicroRNAs - physiology; Molecular Sequence Data; non-coding RNA; Pharmacology/Toxicology; Pharmacy; Research Article; transcription (genetics); 약학; CXCL1; MicroRNA; Human ES cells; Endothelial differentiation
• ISSN: 0253-6269; 1976-3786; 1976-3786
• DOI: 10.1007/s12272-013-0067-9

MicroRNAs (miRNAs) are a class of small noncoding RNAs that negatively regulate gene expression through binding to 3′ untranslated region. We identified and characterized the novel miRNA, miR-7641, in human mesenchymal stem cells. The expression of miR-7641 was downregulated during differentiation from human embryonic stem cells to endothelial cells. The CXCL1, a member of the CXC chemokine family, is known as promoting neovascularization by binding G-protein coupled receptors and is related to endothelial cells biogenesis such as angiogenesis, and it was predicted as target gene of miR-7641 by computerized analysis and the luciferase reporter assay. The miR-7641 significantly suppressed CXCL1 of transcriptional and post-translational levels. These data suggest that miR-7641 might be related with differentiation of human endothelial cells.