A Disintegrin and Metalloproteinase 8 as a Potential Blood Biomarker for Early Diagnosis of Gastric Cancer

• Published in: Yonsei medical journal Vol. 60; no. 8; pp. 713 - 719
• Main Authors: Chung, Hye Won, Kim, Jin Ju, Choi, Jae Il, Lee, Hae Rim, Lim, Jong-Baeck
• Format: Journal Article
• Language: English
• Published: Korea (South) Yonsei University College of Medicine 01.08.2019; 연세대학교의과대학
• Subjects: Original; 의학일반; A disintegrin and metalloproteinase (ADAM 8); diagnostic; early detection; blood biomarker; gastric cancer
• ISSN: 0513-5796; 1976-2437; 1976-2437
• DOI: 10.3349/ymj.2019.60.8.713

We aimed to evaluate the clinical significance of a disintegrin and metalloproteinase 8 (ADAM 8) as a potential blood biomarker for gastric cancer (GC). Blood ADAM 8 was measured by ELISA. Cytokines/chemokines [interleukin-23 (IL-23), stromal cell-derived factor 1α/CXC chemokine ligand 12 (SDF-1α/CXCL12), interleukin-8 (IL-8), and soluble CD40 ligand (sCD40L)] were measured by chemiluminescent immunoassay. They were compared among five groups; normal/gastritis, high-risk, early GC (EGC), advanced GC (AGC) without distant metastasis, and AGC with distant metastasis by one-way analysis of variance in both training (n=80) and validation dataset (n=241). Clinicopathological features of GC and GC-associated cytokines were evaluated for their correlations with blood ADAM 8. To evaluate the diagnostic accuracy to predict GC, receiver operating characteristic (ROC) curve and logistic regression were used. Blood ADAM 8 significantly increased along GC carcinogenesis in both training (ANOVA, <0.001) and validation dataset ( <0.001). It was significantly higher in EGC compared to high-risk (post-hoc Bonferroni, =0.041) and normal ( <0.001). It was also higher in AGC compared with high-risk ( <0.001) and normal ( <0.001) groups. However, no significant difference was found between cancer groups. Blood ADAM 8 was correlated with N-stage (Spearman's correlation, γ =0.320, =0.011), but not with T-stage or M-stage. Pearson's correlations showed blood ADAM 8 was closely correlated with pre-inflammatory cytokines, IL-23 ( =0.036) and SDF-1α/CXCL12 ( =0.037); however, it was not correlated with pro-angiogenic cytokine IL-8 ( =0.313), and sCD40L ( =0.702). ROC curve and logistic regression demonstrated that blood ADAM 8 showed higher diagnostic accuracy (sensitivity, 73.7%; specificity, 86.2%) than CEA (sensitivity, 23.1%; specificity, 91.4%). Combination of ADAM 8 and CEA further increased the diagnostic accuracy to predict GC (sensitivity, 81.8%; specificity, 84.0%). Blood ADAM 8 is a promising biomarker for early detection of GC.