Myomir dysregulation and reactive oxygen species in aged human satellite cells

• Published in: Biochemical and biophysical research communications Vol. 473; no. 2; pp. 462 - 470
• Main Authors: Di Filippo, Ester Sara, Mancinelli, Rosa, Pietrangelo, Tiziana, La Rovere, Rita Maria Laura, Quattrocelli, Mattia, Sampaolesi, Maurilio, Fulle, Stefania
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 29.04.2016
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Aging; Cells, Cultured; elderly; Gene Expression Regulation; homeostasis; Human satellite cells; Humans; Male; microRNA; MicroRNAs - genetics; Muscle Development; muscles; myomiRNAs; people; reactive oxygen species; Reactive Oxygen Species - metabolism; ROS; Sarcopenia; Sarcopenia - genetics; Sarcopenia - metabolism; Satellite Cells, Skeletal Muscle - cytology; Satellite Cells, Skeletal Muscle - metabolism; skeletal muscle; Young Adult; Aging; Sarcopenia; Human satellite cells; myomiRNAs; ROS
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2016.03.030

Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to the age-associated decline in muscle mass, known as sarcopenia. The myogenic potential impairment does not depend on a reduced myogenic cell number, but mainly on their difficulty to complete a differentiation program. The unbalanced production of reactive oxygen species in elderly people could be responsible for skeletal muscle impairments. microRNAs are conserved post-transcriptional regulators implicated in numerous biological processes including adult myogenesis. Here, we measure the ROS level and analyze myomiR (miR-1, miR-133b and miR-206) expression in human myogenic precursors obtained from Vastus lateralis of elderly and young subjects to provide the molecular signature responsible for the differentiation impairment of elderly activated satellite cells.