Analysis of the genetic diversity and differentiation of Fenneropenaeus penicillatus populations using AFLP technology

• Published in: Chinese journal of oceanology and limnology Vol. 30; no. 3; pp. 440 - 445
• Main Author: 张桂玲 曹媛钰 黎中宝 陈锦 赵斌丽 雷光高 王展林
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.05.2012; SP Science Press; Springer Nature B.V
• Subjects: AFLP; Amplification; Amplified fragment length polymorphism; Animal populations; animals; Biology; Breeding; China; Decapoda; Differentiation; Earth and Environmental Science; Earth Sciences; Environmental degradation; Fenneropenaeus penicillatus; Gene flow; genes; Genetic analysis; Genetic diversity; Genetic resources; Genetic variation; loci; Marine; Marine animals; Marine technology; Oceanography; Polymorphism; Population genetics; Population studies; Populations; Selective breeding; Shannon信息指数; Shellfish; Spawning; Spawning grounds; Sustainable use; Technology; 技术分析; 遗传分化系数; 遗传多样性; 遗传资源保护; 野生种群; 长毛对虾; China; ISEW, China, People's Rep., Guangdong Prov., Shenzhen; ISEW, China, People's Rep., Fujian Prov., Dongshan; INW, Japan, Honshu, Ishikawa Prefect., Nanao; China, People's Rep; genetic diversity; genetic differentiation; AFLP
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-012-1125-8

Fenneropenaeus penicillatus （redtail shrimp） is an important marine commercial animal in China. Recently, its resources have been depleted rapidly as a result of, for example, over-exploitation and environmental degradation of spawning grounds. Therefore, we analyzed the genetic diversity and differentiation of nine wild populations of F. penicillatus of China （Ningde, Lianjiang, Putian, Xiamen, Quanzhou, Zhangpu, Dongshan, Nanao, and Shenzhen populations） by amplified fragment .!ength polymorphism （AFLP） technology, to provide genetic information necessary for resource protection, rejuvenation, artificial breeding, and sustainable use of the resource. Eight AFLP primer pairs were used for amplification, and 508 bands were detected among the populations. The results show that the percentage of polymorphic loci （P） ranged from 41.34% to 63.58%; the Nei＇s gene diversity （H） of the populations was 0.119 4-0.230 5; and Shannon＇s Information Index （/） was 0.184 1-0.342 5. These genetic data indicate that the genetic diversity of F. penicillatus was high. The genetic differentiation coefficient （GsT=0.216 2） and gene flow （Nm=1.812 4） show that there was a high level of genetic differentiation and a moderate level of gene flow among populations. More studies on the genetic differentiation mechanism of F. peniillatus along the south-eastem coast of China need to be conducted to find more effective scientific protection strategies for the conservation ofF. penicillatus genetic resources.