Trends and Challenges in Pesticide Resistance Detection

• Published in: Trends in plant science Vol. 21; no. 10; pp. 834 - 853
• Main Authors: R4p (reflection And Research On Resistance To Pesticides), ., Barres, Benoît, Corio-Costet, Marie-France, Debieu, Danièle D., Délye, Christophe, Fillinger-David, Sabine, Grosman, Jacques, Micoud, Annie, Siegwart, Myriam, Walker, Anne-Sophie
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2016; Elsevier
• Subjects: Animals; bioassays; biochemical assays; Biological Assay; diagnosis; Enzyme Assays; Genotyping Techniques; Herbicide Resistance - genetics; Herbicides; Insecta; Insecticide Resistance - genetics; Insecticides; Life Sciences; Mites; molecular detection; monitoring; pesticide; pesticide resistance; Pesticides; plant pests; plant protection; quantification; resistance mechanisms; technology; diagnosis; bioassays; quantification; molecular detection; biochemical assays; pesticide; indicator; méthode de lutte; pathology; produit phytosanitaire; gestion intégrée des ravageurs; vitis vinifera; weed control methods; integrated disease management; indicateur; bioessai; système de production; pest and control product; exchange; échange; maladie
• ISSN: 1360-1385; 1878-4372; 1878-4372
• DOI: 10.1016/j.tplants.2016.06.006

Pesticide resistance is a crucial factor to be considered when developing strategies for the minimal use of pesticides while maintaining pesticide efficacy. This goal requires monitoring the emergence and development of resistance to pesticides in crop pests. To this end, various methods for resistance diagnosis have been developed for different groups of pests. This review provides an overview of biological, biochemical, and molecular methods that are currently used to detect and quantify pesticide resistance. The agronomic, technical, and economic advantages and drawbacks of each method are considered. Emerging technologies are also described, with their associated challenges and their potential for the detection of resistance mechanisms likely to be selected by current and future plant protection methods. Rational and sustainable (bio)pesticide use in a context of increasingly stringent pesticide regulations and the evolution of resistance in agricultural pests is vital to ensure global food security. Monitoring resistance in pest populations is thus crucial and requires accurate, sensitive, and reliable methods. Methods are reviewed for resistance detection in all major pest categories, with a view to facilitating resistance assay development and encouraging exchanges among communities working on different pests. Resistance diagnosis assays should be sufficiently flexible to follow resistance evolution. They should also enable detection of resistant pest genotypes when their frequencies are still low enough to permit adaptation of crop protection strategies to restrict further resistance selection.