Molecular diversity of Enteromorpha from the coast of Yantai： a dual-marker assessment

• Published in: Chinese journal of oceanology and limnology Vol. 31; no. 6; pp. 1210 - 1215
• Main Author: 刘海燕 刘正一 王寅初 赵玉山 秦松
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.11.2013; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Algae; Cladistics; Coastal biotechnology: Reshaping biosphere along coastal line; DNA; Earth and Environmental Science; Earth Sciences; Enteromorpha; Enteromorpha flexuosa; Enteromorpha linza; Enteromorpha prolifera; Geographical distribution; internal transcribed spacers; ITS序列; Marine; Molecular biology; Nucleotide sequence; Nucleotides; Oceanography; Phylogenetics; Phylogeny; ribosomal DNA; Ulva prolifera; 内转录间隔区; 分子多样性; 双标记; 浒苔; 海岸; 烟台; 评估; China; Yantai China; INW, China, People's Rep., Shandong Prov., Shandong Peninsula, Yantai; internal transcribed spacer (ITS); 5SrDNA non-transcribed spacer (NTS); molecular diversity
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-013-3045-7

We collected nine Enteromorpha specimens from the coast of Yantai and evaluated their diversity based on analyses of their ITS （internal transcribed spacer） and 5S rDNA NTS （non-transcribed spacer） sequences. The ITS sequences showed slight nucleotide divergences between Enteromorpha linza and Enteromorpha prolifera. In contrast, multiple highly variable regions were found in the ITS region of Enteromorpha flexuosa. In general, there were more variable sites in the NTS region than in the ITS region in the three species. The variations in 5S rDNA NTS sequences indicated that the molecular diversity of Enteromorpha from the coast of Yantai is very high. However, a phylogenetic tree constructed using 5S rDNA NTS sequence data indicated that genetic differences were not directly related to geographical distribution.