Component analysis of four-part extracts from Chamaecyparis obtusa Endl. by supercritical fluid extraction and anti-inflammatory effect on RAW 264.7cells

• Published in: Journal of exercise rehabilitation Vol. 15; no. 5; pp. 723 - 730
• Main Authors: Kwak, Byeong Mun, Kim, Ee-Hwa, Kim, Yong-Min, Kim, Hee-Tack
• Format: Journal Article
• Language: English
• Published: Korean Society of Exercise Rehabilitation 01.10.2019; 한국운동재활학회
• Subjects: Original; 심리과학; Anti- inflammatory effect; Supercritical fluid extraction; Chamaecyparis obtusa Endl
• ISSN: 2288-176X; 2288-1778
• DOI: 10.12965/jer.1938442.221

The main purpose of this study was to suggest a solution for inflamma-tion caused by urban daily life based on supercritical extraction of Cha-maecyparis obtusa Endl. (C. obtusa). The leaves, cone, wood, and bark of C. obtusa were separately extracted with supercritical extraction. Al-pha-pinene, anti-inflammatory active compound of C. obtusa, was ana-lyzed by electrospray ionization-mass spectrometry, gas chromatogra-phy, and gas chromatography mass spectrometry from each part of ex-tracts. As a result of the reduction rate of nitric oxide in RAW 264.7 cells, the wood extract exhibited the highest reduction rate was about 45% among extracts from four part of C. obtusa. The wood extract from C. obtusa can be developed as a good anti-inflammatory natural raw ma-terial for natural product industry.