Wound-induced expression of the ferulate 5-hydroxylase gene in Camptotheca acuminata

• Published in: Biochimica et biophysica acta Vol. 1760; no. 2; pp. 182 - 190
• Main Authors: Kim, Young Jin, Kim, Dong Gwan, Lee, Sun Hi, Lee, Incheol
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2006
• Subjects: Abscisic Acid - pharmacology; Acrolein - analogs & derivatives; Acrolein - pharmacology; Amino Acid Sequence; Base Sequence; Camptotheca - enzymology; Camptotheca - genetics; Coumaric Acids - pharmacology; Cyclopentanes - pharmacology; Cytochrome P-450 Enzyme System - biosynthesis; Ethylenes - pharmacology; Ferulate 5-hydroxylase; Hydrogen Peroxide - pharmacology; Lignin; Mixed Function Oxygenases - biosynthesis; Molecular Sequence Data; Onium Compounds - pharmacology; Oxylipins; Phenols - pharmacology; Plant Leaves - drug effects; Plant Leaves - enzymology; Plant Roots - enzymology; Plant Stems - enzymology; Plant Stems - genetics; Salicylic Acid - pharmacology; Sequence Alignment; Stress molecule; Transcription, Genetic - drug effects; Wound response; Lignin; Stress molecule; JARE; SA; AVG; C4H; JA; Wound response; F5H; ERE; FA; DPI; Ferulate 5-hydroxylase; MJ
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2005.08.015

The phenylpropanoid pathway plays important roles in plants following exposure to environmental stresses, such as wounding and pathogen attack, which lead to the production of a variety of compounds, including lignin, flavonoids and phytoalexins. Ferulate 5-hydroxylase (F5H) is a cytochrome P450-dependent monooxygenase that catalyses the hydroxylation of ferulic acid, coniferaldehyde and coniferyl alcohol, leading to sinapic acid and syringyl lignin biosynthesis. We isolated F5H cDNA and genomic DNA from Camptotheca acuminata and investigated the expression pattern of the C. acuminata F5H ( CaF5H1) gene in response to wounding. A search against the BLOCKS database of conserved protein motifs indicated that CaF5H1 retains features in common with F5Hs reported from other plants. 5′-flanking region analysis using the PLACE database showed that putative regulatory elements related to various abiotic and biotic stresses, such as drought, wounding, low temperature and pathogens, exist in the 5′-flanking region of CaF5H1. Based upon these analysis results, we investigated the expression pattern of CaF5H1 gene in response to wounding and stress-related molecules. Here, we show that CaF5H1 transcripts accumulated in the leaves in response to mechanical wounding or the application of molecules involved in the stress response, such as ethylene, ABA and hydrogen peroxide (H 2O 2). The application of salicylic acid and diphenylene iodonium (DPI) inhibited the wound-induced expression of CaF5H1. Taken together, we suggest that wound-induced expression of CaF5H1 may be mediated by MJ and H 2O 2 and enhanced phenylpropanoid contents via CaF5H1 maybe function in response to various stresses, including wounding, in plants.