Inflammatory factors gene polymorphism in recurrent oral ulceration

• Published in: Journal of oral pathology & medicine Vol. 42; no. 7; pp. 528 - 534
• Main Authors: Sun, Mao, Fu, Shan-Min, Dong, Guang-Ying, Wu, Dan, Wang, Guo-Xia, Wu, Yuanming
• Format: Journal Article
• Language: English
• Published: Frederiksberg Blackwell Publishing Ltd 01.08.2013; Wiley
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Alleles; Biological and medical sciences; CD95 antigen; Child; Epidermal Growth Factor - analysis; fas Receptor - analysis; Female; Gene Expression Regulation - genetics; gene polymorphisms; Genetic Predisposition to Disease - genetics; Genome, Human - genetics; Genotype; Humans; inflammatory factors; Interferon-gamma - analysis; Interleukin-10 - analysis; Interleukin-12 Subunit p35 - analysis; Interleukin-12 Subunit p40 - analysis; Interleukin-2 - analysis; Interleukin-4 - analysis; Interleukins - genetics; Male; Medical sciences; Middle Aged; Otorhinolaryngology. Stomatology; Polymorphism, Genetic - genetics; recurrent oral ulceration; Stomatitis, Aphthous - genetics; Stomatitis, Aphthous - immunology; susceptibility; Tumor Necrosis Factor-alpha - analysis; Tumor Necrosis Factor-alpha - genetics; Vascular Endothelial Growth Factor A - analysis; Young Adult; Relapse; susceptibility; Gene; Stomatology; Oral administration; ENT; Ulceration; inflammatory factors; gene polymorphisms; recurrent oral ulceration; Polymorphism
• ISSN: 0904-2512; 1600-0714; 1600-0714
• DOI: 10.1111/jop.12048

Background Some inflammatory factors play an important role in recurrent oral ulceration (ROU). The genetics mechanism of expression level of inflammatory factors is not clear in ROU, but from genetics the expression level of inflammatory factors at least partly depend on the gene polymorphisms. Therfore, we decided to investigate inflammatory factors gene polymorphism and its association with the susceptibility of recurrent oral ulceration in Chinese. Methods Genomic DNA was obtained from 42 subjects with recurrent oral ulceration, 86 subjects of healthy control individuals.Genotypes and alleles of 10 genes and 17 polymorphisms sites were analyzed by Mass‐ARRAY Analyzer method. Then, the differences in distribution of each genotype and allele were compared. Results The statistical differences in distribution of TNF‐α (rs1800629 and rs1800630) genotype and allele were observed among the groups with recurrent oral ulceration and healthy control individuals (P < 0.01), while VEGFA (rs1570360, rs833061, and rs2010963), EGF (rs4444903), TNF (rs361525), IL10 (rs1800896, rs1800872), IL2 (rs2069762), IL4 (rs2243250), Fas (rs1800682, rs2234767), IL12A (rs2243115, rs568408), IL12B (rs3212227), and IFNG (rs2430561) showed no statistical differences of genotype and allele in controls as compared to those in patients. Conclusions This study suggests that the TNF‐α (rs1800629 and rs1800630) genotype is an indicator for the susceptibility of recurrent oral ulceration.