Enhanced sympathetic nerve activity induced by neonatal colon inflammation induces gastric hypersensitivity and anxiety-like behavior in adult rats

• Published in: American journal of physiology: Gastrointestinal and liver physiology Vol. 311; no. 1; pp. G32 - G39
• Main Authors: Winston, John H., Sarna, Sushil K.
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.07.2016
• Subjects: Abdominal Pain - etiology; Abdominal Pain - metabolism; Abdominal Pain - physiopathology; Age Factors; Animals; Animals, Newborn; Anxiety; Anxiety - etiology; Anxiety - metabolism; Anxiety - physiopathology; Anxiety - psychology; Behavior, Animal; Colitis - complications; Colitis - metabolism; Colitis - physiopathology; Colon; Colon - innervation; Disease Models, Animal; Hyperalgesia - etiology; Hyperalgesia - metabolism; Hyperalgesia - physiopathology; Inflammation; Male; Mechanotransduction, Cellular; Medical disorders; Nerve Growth Factor - metabolism; Neurogastroenterology and Motility; Norepinephrine - metabolism; Physiology; Pressure; Rats, Sprague-Dawley; Stomach - innervation; Sympathectomy, Chemical; Sympathetic Nervous System - metabolism; Sympathetic Nervous System - physiopathology; Tyrosine 3-Monooxygenase - metabolism; norepinephrine; functional dyspepsia; nerve growth factor; visceral hypersensitivity
• ISSN: 0193-1857; 1522-1547; 1522-1547
• DOI: 10.1152/ajpgi.00067.2016

Gastric hypersensitivity (GHS) and anxiety are prevalent in functional dyspepsia patients; their underlying mechanisms remain unknown largely because of lack of availability of live visceral tissues from human subjects. Recently, we demonstrated in a preclinical model that rats subjected to neonatal colon inflammation show increased basal plasma norepinephrine (NE), which contributes to GHS through the upregulation of nerve growth factor (NGF) expression in the gastric fundus. We tested the hypothesis that neonatal colon inflammation increases anxiety-like behavior and sympathetic nervous system activity, which upregulates the expression of NGF to induce GHS in adult life. Chemical sympathectomy, but not adrenalectomy, suppressed the elevated NGF expression in the fundus muscularis externa and GHS. The measurement of heart rate variability showed a significant increase in the low frequency-to-high frequency ratio in GHS vs. the control rats. Stimulus-evoked release of NE from the fundus muscularis externa strips was significantly greater in GHS than in the control rats. Tyrosine hydroxylase expression was increased in the celiac ganglia of the GHS vs. the control rats. We found an increase in trait but not stress-induced anxiety-like behavior in GHS rats in an elevated plus maze. We concluded that neonatal programming triggered by colon inflammation upregulates tyrosine hydroxylase in the celiac ganglia, which upregulates the release of NE in the gastric fundus muscularis externa. The increase of NE release from the sympathetic nerve terminals concentration dependently upregulates NGF, which proportionately increases the visceromotor response to gastric distention. Neonatal programming concurrently increases anxiety-like behavior in GHS rats.