A Sporolactobacillus -, Clostridium -, and Paenibacillus - Dominant Microbial Consortium Improved Anaerobic RDX Detoxification by Starch Addition

• Published in: Journal of microbiology and biotechnology Vol. 30; no. 6; pp. 839 - 847
• Main Authors: Khan, Muhammad Imran, Yoo, Keunje, Kim, Seonghoon, Cheema, Sardar Alam, Bashir, Safdar, Park, Joonhong
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Microbiology and Biotechnology 28.06.2020; 한국미생물·생명공학회
• Subjects: Anaerobiosis; Biodegradation, Environmental; DNA, Bacterial - genetics; Explosive Agents; Gram-Positive Endospore-Forming Rods - genetics; Gram-Positive Endospore-Forming Rods - metabolism; Microbial Consortia - genetics; Microbial Consortia - physiology; Research article; RNA, Ribosomal, 16S - genetics; Soil Pollutants - chemistry; Soil Pollutants - metabolism; Starch - metabolism; Triazines - chemistry; Triazines - metabolism; 생물학; starch; Sporlactobacillus; Bioremediation; explosives; MiSeq
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1910.10034

In the present study, an anaerobic microbial consortium for the degradation of hexahydro-1,3,5- trinitro-1,3,5-triazine (RDX) was selectively enriched with the co-addition of RDX and starch under nitrogen-deficient conditions. Microbial growth and anaerobic RDX biodegradation were effectively enhanced by the co-addition of RDX and starch, which resulted in increased RDX biotransformation to nitroso derivatives at a greater specific degradation rate than those for previously reported anaerobic RDX-degrading bacteria (isolates). The accumulation of the most toxic RDX degradation intermediate (MNX [hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine]) was significantly reduced by starch addition, suggesting improved RDX detoxification by the co-addition of RDX and starch. The subsequent MiSeq sequencing that targeted the bacterial 16S rRNA gene revealed that the , , and populations were involved in the enhanced anaerobic RDX degradation. These results suggest that these three bacterial populations are important for anaerobic RDX degradation and detoxification. The findings from this work imply that the , , and dominant microbial consortium may be valuable for the development of bioremediation resources for RDX-contaminated environments.