Copy number aberrations in combined hepatocellular carcinoma and cholangiocarcinoma

• Published in: Experimental and molecular pathology Vol. 92; no. 3; pp. 281 - 286
• Main Authors: You, Huey-Ling, Weng, Shao-Wen, Li, Shau-Hsuan, Wei, Yu-Ching, Sheu, Jim Jinn-Chyuan, Chen, Chih-Mei, Huang, Wan-Ting
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.06.2012; Elsevier
• Subjects: Adult; Aged; Aged, 80 and over; Animals; Bile Duct Neoplasms - genetics; Bile Duct Neoplasms - pathology; Bile Ducts, Intrahepatic; Biological and medical sciences; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - pathology; CHC; Cholangiocarcinoma - genetics; Cholangiocarcinoma - pathology; Chromosome Aberrations; Copy number; DNA Copy Number Variations; DNA, Neoplasm - genetics; Female; Gastroenterology. Liver. Pancreas. Abdomen; genes; genetic relationships; genetic variation; Genome-Wide Association Study - methods; hepatoma; Humans; Investigative techniques, diagnostic techniques (general aspects); Liver Neoplasms - genetics; Liver Neoplasms - pathology; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Middle Aged; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Polymerase Chain Reaction; quantitative polymerase chain reaction; Tumors; Whole genome analysis; CC; Copy number; H&E; Whole genome analysis; HCC; AFP; CN; CA 19-9; Q; FFPE; HPC; qPCR; CEA; CHC; Hepatic disease; Hepatocellular carcinoma; Malignant tumor; Biliary tract disease; Liver cancer; Anatomic pathology; Malignant cholangioma; Digestive diseases; Genome; Cancer
• ISSN: 0014-4800; 1096-0945; 1096-0945
• DOI: 10.1016/j.yexmp.2012.01.009

Combined hepatocellular carcinoma and cholangiocarcinoma (CHC) is a rare liver cancer which shares unequivocal features of both hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC). A greater awareness of genetic relationship between HCC and CC components is limited. To help characterize this rare liver neoplasm, we described clinicopathologic features and evaluated copy number (CN) changes in this study. A total of 13 cases of CHC were collected. Four paired HCC and CC components from four cases were first subject to genome-wide analysis. Nine target genes were subsequently selected for further analysis using quantitative polymerase chain reaction. The paired HCC and CC components in each case had a concordant trend of CN gain or loss in these nine genes. However, the magnitude of concordant CN gain or loss was different. There were significant differences of CN copies between HCC and CC in each case. We demonstrate genetic divergence between HCC and CC components in CHC.