Effect of substance P and eledoisin on K + efflux, amylase release and cyclic nucleotide levels in slices of rat parotid gland

• Published in: Biochimica et biophysica acta Vol. 444; no. 3; pp. 704 - 711
• Main Authors: Rudich, Lynn, Butcher, Fred R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 22.10.1976
• Subjects: Amylases - metabolism; Animals; Atropine - pharmacology; Cyclic AMP - metabolism; Cyclic GMP - metabolism; Eledoisin - pharmacology; Female; Kinetics; Parotid Gland - drug effects; Parotid Gland - metabolism; Phentolamine - pharmacology; Potassium - metabolism; Propranolol - pharmacology; Rats; Substance P - pharmacology
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(76)90317-2

The undecapeptides, substance P and eledosin, caused a rapid, concentration-dependent increase in K + efflux and amylase release from parotid tissue slices. The effects were not blocked by β-adrenergic, α-adrenergic, or cholinergic anatagonists. Incubation buffer calcium was required for stimulation of K + efflux and amylase release. The action of the undecapeptides was independent of any effects on parotid cyclic AMP or cyclic GMP levels. Since the actions of the undecapeptides were Ca 2+ dependent and no effects on cyclic nucleotide levels were discerned it was concluded that Ca 2+ plays a primary role in agonist regulation of K + efflux from the parotid.