Inhibitory effects of essential oils from Asteraceae plant against pathogenic fungi of Panax notoginseng

• Published in: Journal of applied microbiology Vol. 130; no. 2; pp. 592 - 603
• Main Authors: Zeng, Z.‐Y., Li, Q.‐Q., Huo, Y.‐Y., Chen, C.‐J., Duan, S.‐S., Xu, F.‐R., Cheng, Y.‐X., Dong, X.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2021
• Subjects: Antifungal activity; Antifungal agents; antifungal properties; Asteraceae; Asteraceae - chemistry; Asteraceae - classification; Asteraceae essential oils; Biological control; Chrysanthemum indicum; Drug Synergism; Essential oils; Fungi; Fungi - classification; Fungi - drug effects; Fungi - growth & development; Fungicides; Fungicides, Industrial - pharmacology; Fusarium oxysporum; Growth rate; Herbal medicine; hymexazol; Hyphae - classification; Hyphae - drug effects; Hyphae - growth & development; In vivo methods and tests; Laggera; Laggera pterodonta; mycelium; Oils & fats; Oils, Volatile - pharmacology; Oxazoles - pharmacology; Panax notoginseng; Panax notoginseng - microbiology; pathogenic fungi; Pesticides; Plant Diseases - microbiology; Plant Diseases - prevention & control; Plant Oils - pharmacology; Pythium aphanidermatum; Root rot; synergism; Synergistic effect; Laggera pterodonta; Panax notoginseng; Asteraceae essential oils; Chrysanthemum indicum; pathogenic fungi
• ISSN: 1364-5072; 1365-2672; 1365-2672
• DOI: 10.1111/jam.14606

Aims Diseases caused by pathogenic fungi was a major constrain in increasing productivity and improving quality of Panax notoginseng. The aim of this research was to evaluate the inhibitory activity of essential oils (EOs) from Asteraceae family, Chrysanthemum indicum and Laggera pterodonta, against pathogenic fungi of P. notoginseng. Methods and Results The antifungal activity was investigated using multiple methods, disclosing that the EOs from C. indicum and L. pterodonta are active against hypha growth of different fungi but with different degrees of potency. Checkerboard testing indicated that the combination of EOs with hymexazol had synergistic effect against Pythium aphanidermatum, and exhibited additive effects against bulk of targeted pathogenic fungi. Besides, we found that the baseline sensitivity of Fusarium oxysporum to L. pterodonta EOs was higher than those of C. indicum by means of mycelium growth rate method. Finally, the practicability of those EOs as plant pesticide was confirmed by in vivo model showing that EOs can significantly inhibit the occurrence of root rot of P. notoginseng caused by F. oxysporum. Conclusion Those studies suggest that the EOs from C. indicum and L. pterodonta had the potential to develop into new pollution‐free pesticides for the protection of precious Chinese herbal medicines. Significance and Impact of the Study This study provided a new way of biological control for overcoming the frequent diseases occurrence of P. notoginseng.