Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig

One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that ar...

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Published inCell transplantation Vol. 32; p. 9636897221107009
Main Authors Kobayashi, Yoshiomi, Shigyo, Michiko, Platoshyn, Oleksandr, Marsala, Silvia, Kato, Tomohisa, Takamura, Naoki, Yoshida, Kenji, Kishino, Akiyoshi, Bravo-Hernandez, Mariana, Juhas, Stefan, Juhasova, Jana, Studenovska, Hana, Proks, Vladimir, Driscoll, Shawn P., Glenn, Thomas D., Pfaff, Samuel L., Ciacci, Joseph D., Marsala, Martin
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.01.2023
Sage Publications Ltd
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ISSN0963-6897
1555-3892
1555-3892
DOI10.1177/09636897221107009

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Summary:One of the challenges in clinical translation of cell-replacement therapies is the definition of optimal cell generation and storage/recovery protocols which would permit a rapid preparation of cell-treatment products for patient administration. Besides, the availability of injection devices that are simple to use is critical for potential future dissemination of any spinally targeted cell-replacement therapy into general medical practice. Here, we compared the engraftment properties of established human-induced pluripotent stem cells (hiPSCs)-derived neural precursor cell (NPCs) line once cells were harvested fresh from the cell culture or previously frozen and then grafted into striata or spinal cord of the immunodeficient rat. A newly developed human spinal injection device equipped with a spinal cord pulsation-cancelation magnetic needle was also tested for its safety in an adult immunosuppressed pig. Previously frozen NPCs showed similar post-grafting survival and differentiation profile as was seen for freshly harvested cells. Testing of human injection device showed acceptable safety with no detectable surgical procedure or spinal NPCs injection-related side effects.
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Yoshiomi Kobayashi is also affilated to The National Medical Center for Spine and Spinal Cord disease, Murayama Medical Center, Gakuen, Musashimurayama city, Tokyo, Japan
Co-first author
ISSN:0963-6897
1555-3892
1555-3892
DOI:10.1177/09636897221107009