Jaboticaba Peel Extract Attenuates Ovariectomy-Induced Bone Loss by Preserving Osteoblast Activity

• Published in: Biology (Basel, Switzerland) Vol. 13; no. 7; p. 526
• Main Authors: Adolpho, Letícia Faustino, Gomes, Maria Paula Oliveira, Freitas, Gileade Pereira, Bighetti-Trevisan, Rayana Longo, Ramos, Jaqueline Isadora Reis, Campeoti, Gabriela Hernandes, Zatta, Guilherme Crepi, Almeida, Adriana Luisa Gonçalves, Tarone, Adriana Gadioli, Marostica-Junior, Mario Roberto, Rosa, Adalberto Luiz, Beloti, Marcio Mateus
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.07.2024
• Subjects: adipocyte; Adipocytes; Adipose tissue; adiposity; animal model; Bisphosphonates; Body weight; Bone density; bone formation; Bone loss; Bone marrow; Bone matrix; bone resorption; Cancellous bone; Cell culture; Cell differentiation; Computed tomography; culture media; Digital cameras; Extracellular matrix; Fractures; Gene expression; genes; Health care expenditures; histology; humans; jaboticaba; Laboratory animals; Mesenchymal stem cells; micro-computed tomography; Mineralization; Oophorectomy; osteoblast; Osteoblastogenesis; Osteoblasts; Osteoporosis; Ovariectomy; Phosphatase; Pilot projects; Plant extracts; Plinia cauliflora; protein synthesis; Software; Therapeutic applications; Womens health; United States > US; Germany; jaboticaba; osteoblast; animal model; osteoporosis; bone loss; adipocyte
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology13070526

Therapies to prevent osteoporosis are relevant since it is one of the most common non-communicable human diseases in the world and the most prevalent bone disorder in adults. Since jaboticaba peel extract (JPE) added to the culture medium enhanced the osteogenic potential of mesenchymal stem cells (MSCs) derived from osteoporotic rats, we hypothesized that JPE prevents the development of ovariectomy-induced osteoporosis. Ovariectomized rats were treated with either JPE (30 mg/kg of body weight) or its vehicle for 90 days, starting 7 days after the ovariectomy. Then, the femurs were subjected to microcomputed tomography and histological analyses, and the osteoblast and adipocyte differentiation of MSCs was evaluated. JPE attenuated ovariectomy-induced bone loss, as evidenced by higher bone volume/total volume and trabecular number, along with lower trabecular separation and bone marrow adiposity. These protective effects of JPE on bone tissue are due to its ability to prevent the imbalance between osteoblast and adipocyte differentiation of MSCs, since, compared with MSCs derived from ovariectomized rats treated with vehicle, MSCs treated with JPE exhibited higher gene and protein expression of osteogenic markers and extracellular matrix mineralization, as well as lower gene expression of adipogenic markers. These data highlight the potential therapeutic use of JPE to prevent osteoporosis.