Expression, purification and characterization of Solanum tuberosum recombinant cytosolic pyruvate kinase

• Published in: Protein expression and purification Vol. 110; pp. 7 - 13
• Main Authors: Auslender, Evgenia L., Dorion, Sonia, Dumont, Sébastien, Rivoal, Jean
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2015
• Subjects: Adenosine Diphosphate - chemistry; Chaperone; Citric Acid - chemistry; Cloning, Molecular; Cytosol - chemistry; Cytosol - enzymology; Enzyme Stability; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Gene Expression; Glycolysis; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Hydrogen-Ion Concentration; Ketoglutaric Acids - chemistry; Kinetics; Molecular Weight; Phosphoenolpyruvate - chemistry; Plant; Plant Proteins - antagonists & inhibitors; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plant Proteins - isolation & purification; Plasmids - chemistry; Plasmids - metabolism; Protein Binding; Pyruvate kinase; Pyruvate Kinase - antagonists & inhibitors; Pyruvate Kinase - biosynthesis; Pyruvate Kinase - genetics; Pyruvate Kinase - isolation & purification; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - isolation & purification; Recombinant protein; Respiratory metabolism; Solanum tuberosum - chemistry; Solanum tuberosum - enzymology; Uridine Diphosphate - chemistry; Glycolysis; Plant; Respiratory metabolism; Recombinant protein; Pyruvate kinase; Chaperone
• ISSN: 1046-5928; 1096-0279; 1096-0279
• DOI: 10.1016/j.pep.2014.12.015

•Potato pyruvate kinase 1 was co-expressed in E. coli with chaperones GroES-GroEL.•Pyruvate kinase 1 solubility and purification protocol were optimized.•The recombinant enzyme was relatively thermostable.•Recombinant potato pyruvate kinase 1 was regulated by Krebs cycle intermediates. The cDNA encoding for a Solanum tuberosum cytosolic pyruvate kinase 1 (PKc1) highly expressed in tuber tissue was cloned in the bacterial expression vector pProEX HTc. The construct carried a hexahistidine tag in N-terminal position to facilitate purification of the recombinant protein. Production of high levels of soluble recombinant PKc1 in Escherichia coli was only possible when using a co-expression strategy with the chaperones GroES-GroEL. Purification of the protein by Ni2 + chelation chromatography yielded a single protein with an apparent molecular mass of 58kDa and a specific activity of 34unitsmg−1 protein. The recombinant enzyme had an optimum pH between 6 and 7. It was relatively heat stable as it retained 80% of its activity after 2min at 75°C. Hyperbolic saturation kinetics were observed with ADP and UDP whereas sigmoidal saturation was observed during analysis of phosphoenolpyruvate binding. Among possible effectors tested, aspartate and glutamate had no effect on enzyme activity, whereas α-ketoglutarate and citrate were the most potent inhibitors. When tested on phosphoenolpyruvate saturation kinetics, these latter compounds increased S0.5. These findings suggest that S. tuberosum PKc1 is subject to a strong control by respiratory metabolism exerted via citrate and other tricarboxylic acid cycle intermediates.