Utilization of cellobiose and other β-D-glucosides in Agrobacterium tumefaciens

• Published in: Research in microbiology Vol. 146; no. 6; pp. 485 - 492
• Main Authors: Marasco, R., Lago, C.T., De Felice, M.
• Format: Journal Article
• Language: English
• Published: Paris Elsevier SAS 1995; Elsevier
• Subjects: Agrobacterium tumefaciens; Agrobacterium tumefaciens - metabolism; Bacterial plant pathogens; beta-Glucosidase - chemistry; beta-Glucosidase - metabolism; Biological and medical sciences; Cellobiose - metabolism; Cellobiose, Agrobacterium tumefaciens, β-Glucosidase, Répression catabolique; Cellobiose, Catabolite repression, β-Glucosidase, Agrobacterium tumefaciens; Cyclic AMP - metabolism; Depression, Chemical; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Glucosides - metabolism; In Vitro Techniques; Phytopathology. Animal pests. Plant and forest protection; Systematics. Structure, properties and multiplication. Genetics; β-D-glucosides, AMP cyclique, Virulence; β-D-glucosides, Cyclic AMP, Virulence; Cellobiose, Catabolite repression, β-Glucosidase, Agrobacterium tumefaciens; β-D-glucosides, Cyclic AMP, Virulence; Cellobiose, Agrobacterium tumefaciens, β-Glucosidase, Répression catabolique; β-D-glucosides, AMP cyclique, Virulence; Plant pathogen; Enzyme; Glucoside; Agrobacterium tumefaciens; Metabolism; Pathogenicity; Regulation(control); β-Glucosidase; Cellulolytic enzyme; Cellobiose; Glycosidases; Bacteria; Hydrolases; Rhizobiaceae; O-Glycosidases; Polysaccharide; Catabolic repression
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/0923-2508(96)80294-4

Agrobacterium tumefaciens strain C58 was able to utilize carbon from cellobiose and some other β-D-glucosides as efficiently as from glucose. β-D-glucoside utilization was partially inducible and the induction was subject to catabolite repression by glucose, independently of the presence of cyclic AMP in the medium. It was also independent of Ti plasmid-encoded functions. β-D-glucosides were hydrolysed by a single, cytoplasmic and constitutively expressed β-glucosidase, which was active on non-phosphorylated substrates and insensitive to glucose inhibition. La souche C58 de Agrobacterium tumefaciens utilise le carbone à partir du cellobiose et d'autres β-D-glucosides avec autant d'efficacité qu'à partir du glucose. L'utilisation de β-D-glucosides est partiellement inductible, et l'induction est sujette à la répression catabolique par le glucose indépendamment de la présence de cAMP dans le milieu. Cette utilisation est aussi indépendante des fonctions codées par le plasmide Ti. Les β-D-glucosides sont hydrolysés par une seule et constitutive β-glucosidase cytoplasmique, active sur les substrats non phosphorylés et insensible à l'inhibition par le glucose.