Generation and characterization of the LINC01405 knockout human embryonic stem cell line

Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains...

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Published inStem cell research Vol. 82; p. 103619
Main Authors Xu, Yaping, Wu, Hongchun, Jiang, Jingxiu, Ye, Lingqun, Hao, Kaili, Han, Kunjun, Hu, Shijun, Lei, Wei, Guo, Zhikun
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.02.2025
Elsevier
Subjects
Cell Differentiation
Cell Line
CRISPR-Cas Systems
Gene Knockout Techniques
Human Embryonic Stem Cells - cytology
Human Embryonic Stem Cells - metabolism
Humans
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
Online AccessGet full text
ISSN1873-5061
1876-7753
1876-7753
DOI10.1016/j.scr.2024.103619

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Summary:Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains human stem cell-like morphology and pluripotency, exhibits a normal karyotype, and can differentiate into cells from all three germ layers. This cell line will be an invaluable model for the research on LINC01405's role in normal development of cardiac and skeletal muscle, and their diseases.
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ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2024.103619