Aiolos Promotes Anchorage Independence by Silencing p66Shc Transcription in Cancer Cells

• Published in: Cancer cell Vol. 25; no. 5; pp. 575 - 589
• Main Authors: Li, Xichuan, Xu, Zhao, Du, Wei, Zhang, Zhenfa, Wei, Yiliang, Wang, Hao, Zhu, Zhiyan, Qin, Litao, Wang, Lin, Niu, Qing, Zhao, Xiulan, Girard, Luc, Gong, Yimei, Ma, Zhenyi, Sun, Baocun, Yao, Zhi, Minna, John D., Terada, Lance S., Liu, Zhe
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.05.2014
• Subjects: Anoikis - genetics; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - mortality; Cell Adhesion - genetics; Cell Adhesion - physiology; Cell Communication - physiology; Cell Line, Tumor; Cell Proliferation; Cell Transformation, Neoplastic - genetics; Chromatin - metabolism; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Silencing; Humans; Ikaros Transcription Factor - metabolism; Lung - metabolism; Lung - pathology; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - mortality; Lymphopoiesis - genetics; Promoter Regions, Genetic; Shc Signaling Adaptor Proteins - genetics; Src Homology 2 Domain-Containing, Transforming Protein 1; Transcription, Genetic
• ISSN: 1535-6108; 1878-3686; 1878-3686
• DOI: 10.1016/j.ccr.2014.03.020

Anchorage of tissue cells to their physical environment is an obligate requirement for survival that is lost in mature hematopoietic and in transformed epithelial cells. Here we find that a lymphocyte lineage-restricted transcription factor, Aiolos, is frequently expressed in lung cancers and predicts markedly reduced patient survival. Aiolos decreases expression of a large set of adhesion-related genes, disrupting cell-cell and cell-matrix interactions. Aiolos also reconfigures chromatin structure within the SHC1 gene, causing isoform-specific silencing of the anchorage reporter p66Shc and blocking anoikis in vitro and in vivo. In lung cancer tissues and single cells, p66Shc expression inversely correlates with that of Aiolos. Together, these findings suggest that Aiolos functions as an epigenetic driver of lymphocyte mimicry in metastatic epithelial cancers. [Display omitted] •Aiolos downregulates multiple cell-cell and cell-matrix adhesion genes•Aiolos epigenetically silences p66Shc and promotes metastatic behavior•p66Shc correlates inversely with Aiolos in human lung tumors•Expression of Aiolos worsens clinical outcome in subjects with lung cancer Li et al. find that the lymphocyte lineage-restricted transcription factor Aiolos is frequently expressed in lung cancers and associates with poor prognosis. Aiolos blocks anoikis and promotes anchorage independence and metastasis in vivo by silencing p66Shc and suppressing adhesion-related genes.