Frequency of HPA-15a and HPA-15b (Gov a/b) Human Platelet Alloantigens in the Croatian Population

• Published in: Archives of medical research Vol. 37; no. 1; pp. 172 - 174
• Main Authors: Tomicic, Maja, Bingulac-Popovic, Jasna, Drazic, Vesna, Hundric-Haspl, Zeljka
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2006
• Subjects: Antigens, CD - genetics; Antigens, CD - immunology; Antiplatelet antibodies; Autoantibodies - immunology; Croatia; Croatian population; Female; Gene Frequency; GPI-Linked Proteins; Human platelet antigens 15a/b frequency; Humans; Isoantibodies - immunology; Isoantigens - genetics; Isoantigens - immunology; Male; Monoclonal antibody-immobilization of platelet antigens; Neonatal alloimmune thrombocytopenia; Neoplasm Proteins - genetics; Neoplasm Proteins - immunology; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Thrombocytopenia - immunology; Croatia; Antiplatelet antibodies; Croatian population; Neonatal alloimmune thrombocytopenia; Human platelet antigens 15a/b frequency; Monoclonal antibody-immobilization of platelet antigens
• ISSN: 0188-4409; 1873-5487
• DOI: 10.1016/j.arcmed.2005.06.001

Human platelet antigen (HPA) genotyping is important for epidemiological studies because the prevalence of particular HPA allotypes differs among various populations and plays a major role in the occurrence of HPA alloimmunization. In Caucasians, antibodies to HPA-1a are the most important causes of neonatal alloimmune thrombocytopenia (NATP). Recent studies suggest that anti-HPA 15a/15b (Gov b, Gov a) might be the most likely candidate antibodies following anti-HPA-1a in inducing NATP. In the present study, HPA-15 system genotype was determined by PCR-SSP method in 279 unrelated subjects from the Croatian population, yielding an HPA-15a and HPA-15b frequency of 0.53 and 0.47, respectively. Retrograde testing for the presence of anti-HPA-15 antibodies by use of MAIPA in 39 frozen serum samples from serologically negative cases of clinically suspect NATP produced negative results. The clinical role of anti-HPA-15 alloantibodies was unable to be confirmed.