Calcium dynamics during trap closure visualized in transgenic Venus flytrap

• Published in: Nature plants Vol. 6; no. 10; pp. 1219 - 1224
• Main Authors: Suda, Hiraku, Mano, Hiroaki, Toyota, Masatsugu, Fukushima, Kenji, Mimura, Tetsuro, Tsutsui, Izuo, Hedrich, Rainer, Tamada, Yosuke, Hasebe, Mitsuyasu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2020
• Subjects: 631/449/1736; 631/449/2653/2658; 631/449/2669; Biomedical and Life Sciences; Calcium - metabolism; Droseraceae - metabolism; Letter; Life Sciences; Physical Stimulation; Plant Leaves - metabolism; Plant Leaves - physiology; Plant Sciences; Plants, Genetically Modified
• ISSN: 2055-0278; 2055-0278
• DOI: 10.1038/s41477-020-00773-1

The leaves of the carnivorous plant Venus flytrap, Dionaea muscipula (Dionaea) close rapidly to capture insect prey. The closure response usually requires two successive mechanical stimuli to sensory hairs on the leaf blade within approximately 30 s (refs. 1 – 4 ). An unknown biological system in Dionaea is thought to memorize the first stimulus and transduce the signal from the sensory hair to the leaf blade 2 . Here, we link signal memory to calcium dynamics using transgenic Dionaea expressing a Ca 2+ sensor. Stimulation of a sensory hair caused an increase in cytosolic Ca 2+ concentration ([Ca 2+ ] cyt ) starting in the sensory hair and spreading to the leaf blade. A second stimulus increased [Ca 2+ ] cyt to an even higher level, meeting a threshold that is correlated to the leaf blade closure. Because [Ca 2+ ] cyt gradually decreased after the first stimulus, the [Ca 2+ ] cyt increase induced by the second stimulus was insufficient to meet the putative threshold for movement after about 30 s. The Ca 2+ wave triggered by mechanical stimulation moved an order of magnitude faster than that induced by wounding in petioles of Arabidopsis thaliana 5 and Dionaea. The capacity for rapid movement has evolved repeatedly in flowering plants. This study opens a path to investigate the role of Ca 2+ in plant movement mechanisms and their evolution. A transgenic Venus flytrap expressing a fluorescent calcium sensor allows real-time live quantification of calcium waves triggered by sensory hair movement. The study suggests that calcium levels represent the molecular basis for the memory effect that requires two stimulations within 30 s.