Immune Response to LinB13, a Lutzomyia Intermedia Salivary Protein Correlates With Disease Severity in Tegumentary Leishmaniasis

• Published in: Clinical infectious diseases Vol. 75; no. 10; pp. 1754 - 1762
• Main Authors: Carvalho, Augusto M, Viana, Sayonara M, Andrade, Bruno B, Oliveira, Fabiano, Valenzuela, Jesus G, Carvalho, Edgar M, de Oliveira, Camila I
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 14.11.2022
• Subjects: Animals; Immunity, Cellular; Immunoglobulin G; Interleukin-10 - metabolism; Leishmania braziliensis; Leishmaniasis, Cutaneous; Leukocytes, Mononuclear; Major; Phlebotomus; Psychodidae; Salivary Proteins and Peptides; Severity of Illness Index; disseminated leishmaniasis; cutaneous leishmaniasis; disease severity; sand fly saliva; Leishmania braziliensis
• ISSN: 1058-4838; 1537-6591; 1537-6591
• DOI: 10.1093/cid/ciac258

Abstract Background We have previously shown that seropositivity to rLinB-13, a salivary protein from Lutzomyia intermedia, predicted sand fly exposure and was associated with increased risk of developing cutaneous leishmaniasis (CL). Methods Here, we investigated the cellular immune response to saliva from Lu. intermedia, using rLinB-13 as a surrogate antigen in naturally exposed individuals presenting positive serology to LinB-13. We also investigated the response to rLinB-13 in leishmaniasis patients, displaying active ulcers and positive PCR for Leishmania braziliensis. Results Peripheral blood mononuclear cells (PBMCs) stimulated in vitro with rLinB-13 secreted elevated levels of interleukin-10 (IL-10), IL-4, IL-1β, IL-1α, IL-6, and chemokines (CCL3, CCL4, CCL5, and CXCL5). CL and disseminated leishmaniasis (DL) patients displayed a significantly higher immunoglobulin G (IgG) response to rLinB-13 compared with healthy subjects, and anti–rLinB-13 IgG was positively correlated with the number of lesions in DL patients. Positive serology to rLinB-13 was also associated with chemotherapy failure. PBMCs from DL patients stimulated with rLINB-13 secreted significantly higher levels of IL-10 and IL-1β compared with CL individuals. Conclusions In this study, we observed an association between humoral and cellular immune response to the sand fly salivary protein rLinB-13 and disease severity in tegumentary leishmaniasis. This study brings evidence that immunity to rLinB-13 influences disease outcome in L. braziliensis infection and results indicate that positive serology to rLinB-13 IgG can be used as a marker of DL, an emerging and severe form of disease caused by L. braziliensis. We observed an association between humoral and cellular immune response to the sand fly salivary protein rLinB-13 and disease severity in tegumentary leishmaniasis. This study brings evidence that immunity to rLinB-13 influences disease outcome in Leishmania braziliensisinfection.