The Secreted Protein MoHrip1 Is Necessary for the Virulence of Magnaporthe oryzae

• Published in: International journal of molecular sciences Vol. 20; no. 7; p. 1643
• Main Authors: Nie, Hai-Zhen, Zhang, Lin, Zhuang, Hui-Qian, Shi, Wen-Jiong, Yang, Xiu-Fen, Qiu, De-Wen, Zeng, Hong-Mei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.04.2019; MDPI
• Subjects: Amino acids; Bioassays; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Expression Regulation, Fungal; Genes, Plant; Infections; Magnaporthe - growth & development; Magnaporthe - metabolism; Magnaporthe - pathogenicity; Magnaporthe - physiology; Mutation - genetics; Oryza - genetics; Oryza - immunology; Oryza - microbiology; Pathogens; Plant Immunity; Plant resistance; Proteins; Signal transduction; Virulence; Magnaporthe oryzae; expression pattern; MoHrip1; virulence
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20071643

Secreted effectors from Magnaporthe oryzae play critical roles in the interaction with rice to facilitate fungal infection and disease development. M. oryzae-secreted protein MoHrip1 can improve plant defense as an elicitor in vitro, however, its biological function in fungal infection is not clear. In this study, we found that the expression of mohrip1 was significantly induced in the stages of fungal penetration and colonization. Although dispensable for the growth and conidiation, MoHrip1 was necessary for the full virulence of M. oryzae. Deletion of mohrip1 remarkably compromised fungal virulence on rice seedlings and even on rice leaves with wounds. Rice sheath inoculation assay further demonstrated the defects of mohrip1-deleted mutants on penetration and proliferation in rice cells. Additionally, compared with WT and complementation strain, the inoculation of mohrip1-deleted mutants induced a higher expression of specific defense related genes and a higher production of specific defensive compounds in rice leaves. These data collectively indicated that MoHrip1 is necessary for fungal penetration and invasive expansion, and further full virulence of rice blast fungus.