Aristoyunnolin H attenuates extracellular matrix secretion in cardiac fibroblasts by inhibiting calcium influx

• Published in: Archives of pharmacal research Vol. 40; no. 1; pp. 122 - 130
• Main Authors: Chen, Shao-rui, Zhang, Wen-ping, Bao, Jing-mei, Cheng, Zhong-bin, Yin, Sheng
• Format: Journal Article
• Language: English
• Published: Seoul Pharmaceutical Society of Korea 01.01.2017; 대한약학회
• Subjects: adults; angiotensin II; Animals; Aristolochia; calcium; Calcium - metabolism; calcium signaling; Calcium Signaling - drug effects; Calcium Signaling - physiology; Cells, Cultured; collagen; Dose-Response Relationship, Drug; extracellular matrix; Extracellular Matrix - drug effects; Extracellular Matrix - secretion; fibroblasts; Fibroblasts - drug effects; Fibroblasts - secretion; fibronectins; Male; Medicine; Myocardium - metabolism; Oriental traditional medicine; Pharmacology/Toxicology; Pharmacy; phosphorylation; protein kinases; Rats; Rats, Sprague-Dawley; Research Article; secretion; Sesquiterpenes - pharmacology; sesquiterpenoids; Western blotting; 약학; Cardiac fibroblasts; Extracellular matrix; Calcium; Aristoyunnolin H
• ISSN: 0253-6269; 1976-3786; 1976-3786
• DOI: 10.1007/s12272-016-0843-4

Aristoyunnolin H is a novel aristophyllene sesquiterpenoid isolated from the traditional Chinese medicine Aristolochia yunnanensis Franch . The present research was designed to explore the anti-fibrotic effects of aristoyunnolin H in adult rat cardiac fibroblasts (CFs) stimulated with angiotensin II (Ang II). Western blot analysis data showed that aristoyunnolin H reduced the upregulation of fibronectin (FN), connective tissue growth factor and collagen I(Col I) production induced by Ang II in CFs. By studying the dynamic intracellular changes of Ca 2+ , we further found that while aristoyunnolin H relieved the calcium influx, it has no effect on intracellular calcium store release. Meanwhile, aristoyunnolin H also inhibited the Ang II-stimulated phosphorylation of Ca 2+ /calmodulin-dependent protein kinase II. In conclusion, aristoyunnolin H may attenuate extracellular matrix secretion in vitro by inhibiting Ang II-induced calcium signaling.