Photo-Cross-Linkers Incorporated into G-Protein-Coupled Receptors in Mammalian Cells: A Ligand Comparison

Capturing the right ligand at the right spot: A well‐balanced system for non‐natural amino acid mutagenesis allows the ligand binding sites of a class II G‐protein coupled receptor to be mapped and distinct binding domains to be identified for different ligands in the native environment of mammalian...

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Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 50; no. 35; pp. 8077 - 8081
Main Authors Coin, Irene, Perrin, Marilyn H., Vale, Wylie W., Wang, Lei
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 22.08.2011
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
Amino acids
Amino Acids - chemistry
Animals
Binding Sites
genetic code
HEK293 Cells
Humans
Ligands
Mammals
Mutagenesis, Site-Directed
non-natural amino acids
photo-cross-linking
photoaffinity labeling
Photoaffinity Labels
Rats
Receptors, G-Protein-Coupled - chemistry
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - metabolism
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
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ISSN1433-7851
1521-3773
1521-3773
DOI10.1002/anie.201102646

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Summary:Capturing the right ligand at the right spot: A well‐balanced system for non‐natural amino acid mutagenesis allows the ligand binding sites of a class II G‐protein coupled receptor to be mapped and distinct binding domains to be identified for different ligands in the native environment of mammalian cells.
Bibliography:NIH - No. 1DP2OD004744-01; No. P30CA014195
National Institute of Diabetes and Digestive and Kidney Disease - No. DK026741-31
ArticleID:ANIE201102646
CIRM - No. RN1-00577-1
istex:288667A0D934B0F25FBC7511F19C0DAAB321F102
Deutsche Forschungsgemeinschaft - No. CO822/1-1
I.C. gratefully acknowledges a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft (CO822/1-1). M.H.P. and W.W.V. acknowledge the support of the National Institute of Diabetes and Digestive and Kidney Disease (DK026741-31) and the Clayton Medical Research Foundation. W.W.V. is a senior CMRF investigator. L.W. thanks support from CIRM (RN1-00577-1) and NIH (1DP2OD004744-01, P30CA014195). We thank Dr. J. Rivier for peptide synthesis and helpful discussions and J. Vaughan for excellent technical assistance.
Clayton Medical Research Foundation
ark:/67375/WNG-RQCH7V4X-W
I.C. gratefully acknowledges a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft (CO822/1‐1). M.H.P. and W.W.V. acknowledge the support of the National Institute of Diabetes and Digestive and Kidney Disease (DK026741‐31) and the Clayton Medical Research Foundation. W.W.V. is a senior CMRF investigator. L.W. thanks support from CIRM (RN1‐00577‐1) and NIH (1DP2OD004744‐01, P30CA014195). We thank Dr. J. Rivier for peptide synthesis and helpful discussions and J. Vaughan for excellent technical assistance.
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ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201102646