Screening and isolation of the algicidal compounds from marine green alga Ulva intestinalis

• Published in: Chinese journal of oceanology and limnology Vol. 34; no. 4; pp. 781 - 788
• Main Author: 孙雪 金浩良 张琳 胡伟 李亚鹤 徐年军
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.07.2016; Springer Nature B.V
• Subjects: 2-脱氧葡萄糖; Acetates; Acetic acid; Akashiwo; Algae; algal blooms; Algicides; Aquatic plants; Biological control; biological control agents; Biological effects; Chemistry; China; Chondria; Chondria crassicaulis; coasts; Earth and Environmental Science; Earth Sciences; Ethanol; Ethyl acetate; Glucose; Gracilariopsis lemaneiformis; Grateloupia; growth retardation; Heterosigma akashiwo; inhibitory concentration 50; macroalgae; Marine; Marine ecology; microalgae; Oceanography; Palmitic acid; Phytoplankton; Prorocentrum; Prorocentrum micans; Red tide; Red tides; screening; Seaweeds; Ulva; Ulva intestinalis; Water pollution; 乙酸乙酯提取物; 分离; 活性化合物; 海洋绿藻; 物质; 筛选; 肠组织; China; red tide algae; inhibitory effect
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-016-4383-z

Twenty species of seaweed were collected from the coast of Zhejiang, China, extracted with ethanol, and screened for algicidal activity against red tide microalgae H eterosigma akashiwo and Prorocentrum micans. Inhibitory eff ects of fresh and dried tissues of green alga U lva intestinalis were assessed and the main algicidal compounds were isolated, purifi ed, and identifi ed. Five seaweed species, U. intestinalis, U. fasciata, Grateloupia romosissima, Chondria crassicaulis, and Gracilariopsis lemaneiformis, were investigated for their algicidal activities. Fresh tissues of 8.0 and 16.0 mg/m L of U. intestinalis dissolved in media signifi cantly inhibited growth of H. akashiwo and P. micans, respectively. Dried tissue and ethyl acetate（Et OAc） extracts of U. intestinalis at greater than 1.2 and 0.04 mg/m L, respectively, were fatal to H. akashiwo, while its water and Et OAc extracts in excess of 0.96 and 0.32 mg/m L, respectively, were lethal to P. micans. Three algicidal compounds in the Et OAc extracts were identifi ed as 15-ethoxy-（6z,9z,12z）-hexadecatrienoic acid（I）,（6E,9E,12E）-（2-acetoxy- β- D-glucose）-octadecatrienoic acid ester（II） and hexadecanoic acid（III）. Of these, compound II displayed the most potent algicidal activity with IC50 values of 4.9 and 14.1 μg/m L for H. akashiwo and P. micans, respectively. Compound I showed moderate algicidal activity with IC50 values of 13.4 and 24.7 μg/m L for H. akashiwo and P. micans, respectively. These fi ndings suggested that certain macroalgae or products therefrom could be used as ef fective biological control agents against red tide algae.