Characterization and antioxidant activities of marine pepsin soluble collagen from the skin of yellow goosefish Lophius litulon

• Published in: Chinese journal of oceanology and limnology Vol. 35; no. 3; pp. 634 - 644
• Main Author: 郑斌 相兴伟 周宇芳 杨会成 罗红宇 廖妙飞 闻正顺
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.05.2017; Springer Nature B.V
• Subjects: Aging (artificial); Anions; antioxidant activity; Antioxidants; Biology; Catalase; Collagen; Collagen (type I); D-Galactose; Denaturation; Earth and Environmental Science; Earth Sciences; Fish; Fourier transform infrared spectroscopy; Fourier transforms; Free radicals; Galactose; Glutathione; Glutathione peroxidase; Helices; Hydroxyl radicals; Hydroxyproline; imino acids; Infrared analysis; Infrared spectra; Infrared spectroscopy; Lophius americanus; Lophius litulon; malondialdehyde; Marine; Oceanography; Pepsin; Peptides; Peroxidase; Proteins; Residues; Scavenging; Sodium chloride; Solubility; superoxide anion; Superoxide anions; Superoxide dismutase; temperature; 傅立叶变换红外光谱; 抗氧化活性; 海洋; 皮肤老化; 胃蛋白酶; 胶原蛋白; 谷胱甘肽过氧化物酶; 超氧化物歧化酶; yellow goosefish; pepsin soluble collagen; antioxidant activity
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-017-5377-1

Characteristics and antioxidant activities of pepsin-soluble collagen （PSC） from yellow goosefish （Lophius litulon） skins were investigated. PSC was characterized as a type I collagen, and its imino acid content was 193 residues/1 000 residues. PSC＇s denaturation temperature was -17.56℃ and Fourier transform infrared spectra confirmed the presence of triple helices. Solubility analysis showed good solubility at acidic pH （1-6） or low NaCl concentrations （≤2%）. PSC showed scavenging activity against hydroxyl radicals and superoxide anions in a concentration-dependent manner. Furthermore, PSC could protect D-galactose-induced skin aging by significantly controlling malondialdehyde formation and improving the activity of superoxide dismutase, glutathione peroxidase, catalase, glutathione, and hydroxyproline. PSC may be a promising antioxidant in appropriate applications.