Interspecific competition and allelopathic interaction between Karenia mikimotoi and Dunaliella salina in laboratory culture

• Published in: Chinese journal of oceanology and limnology Vol. 34; no. 2; pp. 301 - 313
• Main Author: 何冬 刘娇 郝锵 冉莉华 周斌 唐学玺
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.03.2016; Springer Nature B.V
• Subjects: Algae; Allelochemicals; Allelopathy; Cell culture; Chemical interactions; color; culture filtrates; Dunaliella salina; Earth and Environmental Science; Earth Sciences; Exudates; Exudation; Filtrate; High temperature; Interspecific; interspecific competition; Irradiance; Karenia; Karenia mikimotoi; Laboratory culture; light intensity; Marine; Microalgae culture; Oceanography; S2 Adaptation and Evolution to Special Environment of Coastal Zone; Salina; Sediments; temperature; 化学相互作用; 化感物质; 实验室培养; 杜氏盐藻; 植物化感作用; 生长特性; 种间竞争; 细胞粘附; allelopathy; cell-free filtrate; bi-algal culture
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-016-4320-1

Algal allelopathy is a manifold ecological/physiological phenomenon that is focused on chemical interactions and autotoxicity. We investigated the allelopathic interactions between Karenia mikimotoi and Dunaliella salina in laboratory cultures based on different temperature （15℃, 20℃, and 25℃） and lighting （40,80, and 160 umol/（m2·s）） conditions. The growth of D. salina in bi-algae culture （1：1 size/density） was significantly restrained. The results of cell-free filtrate culture indicate that direct cell-to- cell contact was not necessary in interspecific competition. Further experimental results demonstrated that allelochemicals released from K. miMmotoi were markedly influenced by both temperature （P=0.013） and irradiance （P=0.003）, resulting in different growth characteristics olD. salina in filtrate mediums. Compared with the plateau period, K. mikimotoi exudates in the exponential phase had a stronger short-term inhibition effect on D. salina in normal conditions. A clear concentration-dependent relationship was observed in the effect of allelochemicals released from K. mikimotoi with low-promoting and high-repressing effects on D. Salina in a short time-scale. In addition, allelopathic substances remain stable and effective under high temperature and pressure stress. Many flocculent sediments adhering with D. salina cells were observed in all filtrate mediums, while the quantity and color depended on the original culture conditions.