Microgravity and Cell Adherence

• Published in: International journal of molecular sciences Vol. 21; no. 6; p. 2214
• Main Author: Bauer, Johann
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.03.2020; MDPI
• Subjects: Antigens; Apoptosis; Binding sites; Breast cancer; Cancer; Cell Adhesion; Cell adhesion & migration; Conflicts of interest; Cyclin-dependent kinases; Enzymes; Extracellular matrix; Gene expression; Humans; Investigations; Kinases; Metastasis; Phosphatase; Proteins; Retina; Retinoblastoma; Weightlessness; Wound healing
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21062214

In these cells, the intercellular adhesion molecule 3 was reduced when they were cultured on a random positioning machine (RPM) for 96 h. Also, in lung cancer cells, incubation on an RPM induced apoptosis within 24 h [14]. After culturing healthy human umbilical vein endothelial cells (HUVEC) or malignant human breast cancer cells in a rotating wall vessel (RWVS), the authors observed that adhesion strength of the cells to the underlay decreased so that more cells could be exfoliated under equal treatment from slips mounted on the RWVS than from equal slips used for culturing these cells at 1g normal gravity. [...]the paper strongly suggests a link between the adhesion strength to the extracellular matrix via integrin β1 or to neighboring cells via e-cadherin and an induction of EMT. Abbreviations ASAP1 PTK2/FAK1 Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 Focal adhesion kinase 1 Bcar1 Breast cancer anti-estrogen resistance protein 1 CAV1 Caveolin-1 CD44 CD44 antigen CDKN2A Cyclin-dependent kinase inhibitor 2A CXCR4 C-X-C chemokine receptor type 4 ECM Extracellular matrix EMT epithelial-mesenchymal transition ERK1 Mitogen-activated protein kinase 3 HUVEC cGMP Umbelical vein endothelial cells Cyclic guanosine monophosphate ICAM1 Intercellular adhesion molecule 1 NEU1 Sialidase 1 /Neuraminidase 1 PRKCA Protein kinase C alpha type PRP Platelet rich plasma PTEN Phosphatidylinositol 3,4,5-triphossphate 3-phosphatase and dual-specific protein phosphatase PTEN RAF1 RAF proto-oncogene serin/threonine-protein kinase RB1 Retinoblastoma-associated protein RPM Random positioning machine RWVS Rotating Wall Vessel Snail, Zinc finger protein SNAI1 ST6GAL1 Beta-galactoside alpha-2,6-sialyltransferase 1 TP53 Cellular tumor antigen p53 Twist Twist related protein ZEB1 Zinc finger E-box-binding homeobox 1 1.