Phytoactinopolyspora limicola sp. nov., an alkaliphilic actinomycete isolated from a soda alkali-saline soil

• Published in: Archives of microbiology Vol. 203; no. 4; pp. 1367 - 1374
• Main Authors: Wei, Wei, Wang, Kaili, Hu, Xinyu, Yang, Sai, Zeng, Chunlin, Hou, Zhaozhi, Liu, Shenkui, Cui, Henglin, Zhu, Lin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2021; Springer Nature B.V
• Subjects: Actinobacteria - chemistry; Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - physiology; Algorithms; Archives & records; Base Composition; Biochemistry; Biomass; Biomedical and Life Sciences; Biotechnology; Carbon; Cell Biology; China; Deoxyribonucleic acid; Diagnostic systems; Diaminopimelic Acid - analysis; DNA; DNA, Bacterial - genetics; Ecology; Fatty acids; Fatty Acids - analysis; Gene sequencing; Genes; Genome, Fungal - genetics; Genomes; Hybridization; Hydrolysates; Life Sciences; Mannose; Menaquinones; Microbial Ecology; Microbiology; Microscopy; Morphology; New species; Nucleic Acid Hybridization; Nucleotides; Original Paper; pH effects; Phospholipids - chemistry; Phylogenetics; Phylogeny; Physiology; Rhamnose; RNA, Ribosomal, 16S - genetics; rRNA 16S; Saline soils; Sequence Analysis, DNA; Sodium; Sodium chloride; Software packages; Soil - chemistry; Soil Microbiology; Sugar; Sugars - analysis; Vitamin K 2 - analysis; China; Soda alkali-saline soil; Polyphasic taxonomy; Draft genome; sp. nov; Phytoactinopolyspora limicola sp. nov
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-020-02134-0

An alkaliphilic actinomycete, designated HAJB-30  T , was isolated from a soda alkali-saline soil in Heilongjiang, Northeast China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HAJB-30  T was most closely related to type strains of the genus Phytoactinopolyspora with sequence similarities ranging between 93.5 and 98.9%. Strain HAJB-30  T grew at pH 8.0–11.0 (optimum pH 9.5–10.0) and in the presence of 0–7.0% NaCl (optimum 1.0–3.0%). Whole-cell hydrolysates of the isolate contained LL-diaminopimelic acid as the diagnostic diamino acid and mannose and rhamnose as diagnostic sugars. The major fatty acids identified were iso-C 14:0 , iso-C 15:0 , anteiso-C 15:0 , iso-C 16:0 and anteiso-C 17:0 , while the menaquinone was MK-9(H4). The genome (6,589,901 bp), composed of 50 contigs, had a G + C content of 66.8%. Out of the 6074 predicted genes, 6020 were protein-coding genes, and 54 were ncRNAs. Digital DNA–DNA hybridization (dDDH) estimation and average nucleotide identity (ANI) of strain HAJB-30  T against genomes of the type strains of related species in the same family ranged between 19.7 and 22.0% and between 71.5 and 76.8%, respectively. From these results, it was concluded that strain HAJB-30  T possesses sufficient characteristics differentiated from all recognized Phytoactinopolyspora species, it is considered to be a novel species for which the name Phytoactinopolyspora limicola sp. nov. is proposed. The type strain is HAJB-30  T (= CGMCC 4.7591  T , = JCM 33694  T ).