Co-exposure to Environmentally Relevant Levels of Molybdenum and Cadmium Induces Oxidative Stress and Ferroptosis in the Ovary of Ducks Co-exposure to Environmentally Relevant Levels of Molybdenum and Cadmium Induces Oxidative Stress and Ferroptosis in the Ovary of Ducks

• Published in: Biological trace element research Vol. 203; no. 1; pp. 374 - 383
• Main Authors: Wang, Dianyun, Zhang, Caiying, Guo, Huiling, Cui, Ting, Pu, Wenjing, Huang, Bingyan, Zhu, Jiamei, Dai, Xueyan
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.01.2025; Springer Nature B.V
• Subjects: Aquatic birds; Biochemistry; Biological stress; Biomedical and Life Sciences; Biotechnology; Cadmium; diet; ducks; Exposure; Ferroptosis; Ferrous ions; Hydrogen peroxide; Life Sciences; Molybdenum; NRF2 protein; Nutrition; Oncology; Ovaries; Oxidative stress; protein content; Proteins; reproductive toxicology; Toxicity; Waterfowl; Cadmium; Oxidative stress; Ovary; Ferroptosis; Molybdenum
• ISSN: 0163-4984; 1559-0720; 1559-0720
• DOI: 10.1007/s12011-024-04144-1

Excessive doses of molybdenum (Mo) and cadmium (Cd) have toxic effects on animals. Nevertheless, the reproductive toxicity elicited by Mo and Cd co-exposure remains obscure. To evaluate the co-induce toxic impacts of Mo and Cd on ovaries, 8-day-old 40 healthy ducks were stochastically distributed to four groups and were raised a basal diet supplemented with Cd (4 mg/kg Cd) and/or Mo (100 mg/kg Mo). In the 16th week, ovary tissues were gathered. The data revealed that Mo and/or Cd decreased GSH content, CAT, T-SOD, and GSH-Px activities and increased MDA and H 2 O 2 levels. Moreover, there was a significant decrease in nuclear Nrf2 protein level and its related downstream factors, while cytoplasmic Nrf2 protein level showed a substantial increase. Additionally, a marked elevation was observed in ferrous ion content and TFRC, GCLC, SLC7A11, ACSL4, and PTGS2 expression levels, while FTH1, FTL1, FPN1, and GPX4 expression levels were conversely reduced. These indicators exhibited more marked changes in the joint exposure group. In brief, our results announced that Mo and/or Cd resulted in oxidative stress and ferroptosis in duck ovaries. Synchronously, the Cd and Mo mixture intensified the impacts.