Sulfide-based ATP production in Urechis unicinctus

• Published in: Chinese journal of oceanology and limnology Vol. 28; no. 3; pp. 521 - 526
• Main Author: 马卓君 包振民 王思锋 张志峰
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science Press 01.05.2010; Springer Nature B.V
• Subjects: Adenosine triphosphatase; adenosine triphosphate; antimycin A; ATP; ATP合成; Biochemistry; Biology; Cyanides; Earth and Environmental Science; Earth Sciences; electron transfer; Electron transport; Hindgut; Inhibitor drugs; Inhibitors; Marine; Marine biology; Mitochondria; Oceanography; Piscicides; Rotenone; Substrate inhibition; Sulfides; Sulphides; tissues; Urechis unicinctus; Worms; 三磷酸腺苷; 呼吸抑制剂; 电子传递途径; 硫化物; ATP production; sulfide oxidation; inhibitor; mitochondria
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-010-9104-4

We measured sulfide-based ATP production by isolated mitochondria from four tissues of Urechis unicinctus and the effects of inhibitors of respiratory complexes on ATP production were evaluated. The results show that these mitochondria could oxidize sulfide to produce ATP. The yield of sulfide-stimulated ATP varied from 5 nmol ATP/min/mg to 90 nmol ATP/min/mg according to the sulfide concentration and the source of the mitochondria. The maximum ATP synthesis occurred in hindgut mitochondria using 5 μmol/L sulfide as a substrate. The effects of inhibitors （Rotenone, Antimycin A, Cyanide, and Salicylhydroxamic acid） on mitochondrial ATP production varied with the source of the mitochondria. Our results indicate that sulfide-based ATP production and the associated electron transport pathway are tissue-specific in U. unicinctus.