Development of a 9-locus X-STR Multiplex PCR System for Genetic Analysis of Three Ethnic Populations in China

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 35; no. 2; pp. 183 - 187
• Main Author: 黄代新 马红杜 杨荣芝 易少华 刘宇轩 胡清清
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.04.2015; Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Kunming People's Procuratorate, Kunming 650224, China
• Subjects: Base Sequence; China; Chromosomes, Human, X; DNA Primers; Ethnic Groups - genetics; Hardy-Weinberg平衡; Humans; Medicine; Medicine & Public Health; Microsatellite Repeats - genetics; Multiplex Polymerase Chain Reaction - methods; STR基因座; 中国汉族; 人群; 复合扩增; 复用系统; 多重聚合酶链反应; 遗传分析; China; multiplex PCR; X-STR; polymorphism; population
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-015-1408-3

X-chromosome short tandem repeats（X-STR） analysis has been confirmed to be effective for kinship testing such as in deficiency paternity cases. The aim of this study was to develop a new multiplex polymerase chain reaction（PCR） system that can simultaneously amplify 9 X-STR loci（GATA172D05, DXS10159, DXS6797, HPRTB, DXS10079, DXS6789, DXS9895, DXS10146 and GATA31E08） in the same PCR reaction, and to obtain the database of the 9 X-STR loci in three ethnic populations in China. The genetic data of 815（404 females and 411 males） unrelated Han Chinese from Hubei province, and Yi and Zhuang Chinese from Yunnan province were analyzed by using this multiplex system. The results showed that a total of 93 alleles for all these loci were found, and 7 to 20 alleles for each locus were observed. All of the analyzed loci were in agreement with Hardy-Weinberg equilibrium after Bonferroni correction in the three studied populations. The polymorphism information content（PIC） and power of discrimination（PD） in females were 0.6566–0.8531 and 0.8639–0.9684, respectively. Pairwise comparisons of allele frequency distribution showed significant differences in the most of these loci between different populations. The results indicate that this multiplex system is very useful for forensic analysis of different ethnic populations in China.