Catabolite repression of the xylanase gene (xynA) expression in Bacillus stearothermophilus No. 236 and B. subtilis

• Published in: Bioscience, biotechnology, and biochemistry Vol. 63; no. 12; pp. 2053 - 2058
• Main Authors: Cho, S.G. (Korea Univ., Seoul (Korea R.)), Choi, Y.J
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.12.1999; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: BACILLUS STEAROTHERMOPHILUS; BACILLUS SUBTILIS; Bacillus subtilis - enzymology; Bacteriology; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; carbon; CATABOLISM; CATABOLISME; CATABOLISMO; catabolite repression; CLONACION MOLECULAR; CLONAGE MOLECULAIRE; Cloning, Molecular; Endo-1,4-beta Xylanases; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Gene Expression Regulation, Enzymologic - drug effects; genes; Genetics; Geobacillus stearothermophilus; Geobacillus stearothermophilus - enzymology; glucose; Glucose - pharmacology; HIDROLASAS; HYDROLASE; HYDROLASES; Microbiology; Mission oriented research; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; nucleotide sequences; nucleotides; plasmids; RECOMBINACION; RECOMBINAISON; RECOMBINATION; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; XILANOS; xylanase; xylanases; XYLANE; XYLANS; Xylosidases - biosynthesis; Xylosidases - genetics; xynA; Recombinant microorganism; Enzyme; Bacillus subtilis; Endo-1,4-β-xylanase; Bacillaceae; Gene expression; Regulation(control); Bacillales; Glycosidases; Bacillus stearothermophilus; Deletion; Bacteria; Hydrolases; Regulatory sequence; O-Glycosidases; Hybrid gene; Catabolic repression
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.63.2053

Catabolite repression of the Bacillus stearothermophilus No.236 xynA gene, encoding an extracellular xylanase, was investigated in this work. Expression of the xynA gene in the B. stearothermophilus strain was found to be subject to glucose catabolite repression, and the level of repression was about 50-fold when the relative amounts of xynA transcript synthesized on different carbon sources were analyzed. The experiments with the B. subtilis MW15 strains carrying plasmids containing the xynA::aprA fusion gene showed that the cloned xynA gene did not require any specific carbon source for its induction. Nevertheless, the expression of the cloned gene was repressed by the presence of glucose. From the nucleotide sequence of the cloned xynA gene, we found two potential catabolite responsive elements (cre) within its reading frame region (cre-1: nucleotides + 160 to + 173 and cre-2: +173 to +186). Furthermore, by using various deletion derivatives of the xynA::aprA fusion plasmid (pMGW23), we suggested that only the cre-1 element might play a role in the glucose catabolite repression. Repression level of the xynA gene expression in the recombinant B. subtilis strain was estimated to be about 3-fold by analysis of the amounts of xynA transcript