Interferon-γ Alters the Immune-related miRNA Expression of Microvesicles Derived from Mesenchymal Stem Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 37; no. 2; pp. 179 - 184
• Main Author: 赵爱琪 谢慧 林生彦 雷倩 任文祥 高飞 郭豪 郭安源 陈智超 王红祥
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.04.2017; Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China%Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Hematology, Wuhan Central Hospital, Wuhan 430022, China
• Subjects: Cell-Derived Microparticles - drug effects; Cell-Derived Microparticles - genetics; Cells, Cultured; Computational Biology - methods; Gene Expression Regulation - drug effects; Gene Regulatory Networks; Humans; IFN-γ; Interferon-gamma - pharmacology; Medicine; Medicine & Public Health; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - immunology; MicroRNAs - genetics; miRNA; MSCs; Oligonucleotide Array Sequence Analysis; Signal Transduction; 免疫抑制作用; 免疫相关; 干扰素-γ; 微泡; 骨髓间充质干细胞; microRNAs; interferon gamma; microvesicles; mesenchymal stem cells
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-017-1712-1

Increasing studies have demonstrated that interferon gamma（IFN-γ）,which serves as a critical inflammatory cytokine,is essential to induce the immunosuppressive effects of mesenchymal stem cells（MSCs）.However,the mechanisms underlying the enhanced immunosuppressive effects of IFN-γ-stimulated MSCs（γMSCs） are not fully understood.MSC-derived microvesicles（MSC-MVs） have been viewed as potential pivotal mediators of the immunosuppressive effects of MSCs.Moreover,micro RNAs（miR NAs） are important regulators of immunological processes and can be shuttled from cell to cell by MVs.The aim of our study was to analyze the the mi RNA expression signature of MVs derived from γMSCs（γMSC-MVs）,which may provide better understanding of the immunosuppressive property of their parent cells.Through mi RNA microarray and bioinformatics analysis,we found 62 significantly differentially expressed miR NAs（DEMs） in γMSC-MVs compared with MSC-MVs.And the potential target genes and signaling pathways regulated by DEMs were predicted and analyzed.Interestingly,many DEMs and predicted signaling pathways had been demonstrated to be involved in immunoregulation.Furthermore,the network between immunoregulation-related pathways and relevant DEMs was constructed.Collectively,our research on the mi RNA repertoires of γMSC-MVs not only provides new perspectives into the mechanisms underlying the enhanced immunosuppressive property of γMSCs,but also paves the way to clinical application of these potent organelles in the future.