Distinct Expression of Chemokine-like Factor 1 in Synovium of Osteoarthritis, Rheumatoid Arthritis and Ankylosing Spondylitis

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 36; no. 1; pp. 70 - 76
• Main Author: 陶可 唐旭 王斌 李儒军 张宝庆 林剑浩 李虎
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.02.2016; Peking University Health Science Center,Beijing 100083,China%Arthritis Clinical and Research Center,Peking University People's Hospital,Beijing 100044,China; Arthritis Clinical and Research Center,Peking University People's Hospital,Beijing 100044,China
• Subjects: Adult; Arthritis, Rheumatoid - metabolism; Biomarkers - metabolism; Case-Control Studies; Chemokines - genetics; Chemokines - metabolism; Female; Humans; Male; MARVEL Domain-Containing Proteins - genetics; MARVEL Domain-Containing Proteins - metabolism; Medicine; Medicine & Public Health; Middle Aged; Osteoarthritis - metabolism; Receptors, CCR4 - genetics; Receptors, CCR4 - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Spondylitis, Ankylosing - metabolism; Synovial Fluid - metabolism; 临床; 医学; 症状; 诊断; CRP; chemokine-like factor 1; ESR; osteoarthritis; ankylosing spondylitis; D-dimer; CCR4; rheumatoid arthritis
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-016-1544-4

Chemokine-like factor 1（CKLF1） is a newly cloned chemotactic cytokine with CCR4 being its functional receptor. Recent evidence demonstrates a role of CKLF1 in arthritis. The aim of this study was to quantify the expression of CKLF1 as well as assess the correlation between CKLF1 and plasma acute-phase markers. Synovium was obtained from 16 osteoarthritis（OA）, 15 rheumatoid arthritis（RA） and 10 ankylosing spondylitis（AS） patients undergoing total joint arthroplasty, with other 11 patients treated for meniscal tears during sport accidents serving as normal controls. Levels of CKLF1 and CCR4 m RNA were detected by q RT-PCR, and the expression of CKLF1 was investigated by immunohistochemistry staining, subsequently analyzed with semiquantitative scores. Plasma acute-phase markers of inflammation were determined by ELISA. CKLF1 was found with a particularly up-regulated expression in synovim from AS and RA patients, and CCR4 m RNA levels increased in RA patients, not in OA or AS patients. Elevated levels of plasma markers of inflammation including CRP, ESR and Ddimer were observed in RA. Further, significantly positive correlations between relative expression levels of CKLF1 and CRP/ESR in RA patients and a positive correlation between CKLF1 and ESR in AS patients were found. There was no detectable correlation between CKLF1 and plasma D-dimer. This study confirms an increased but different level of CKLF1 in RA, OA and AS patients, all significantly higher than that in controls. Additionally, the significant positive correlations between CKLF1 levels and CRP/ESR in RA and between CKLF1 and ESR suggest that CKLF1 might contribute to the inflammation state and clinical symptoms in these rheumatic diseases. Further studies are required to investigate the utility of targeting specific CKLF1 for symptom control or disease modification in RA and AS.