Analysis of serum adenosine deaminase (ADA) and ADA1 and ADA2 isoenzyme activities in HIV positive and HIV–HBV co-infected patients

• Published in: Clinical biochemistry Vol. 44; no. 12; pp. 980 - 983
• Main Authors: Khodadadi, Iraj, Abdi, Mohammad, Ahmadi, Abbas, Wahedi, Mohammad Saleh, Menbari, Shahoo, Lahoorpour, Fariba, Rahbari, Rezgar
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.08.2011; Elsevier
• Subjects: Adenosine deaminase; Adenosine Deaminase - blood; Adult; Alanine Transaminase - blood; Aspartate Aminotransferases - blood; Biological and medical sciences; Case-Control Studies; CD4 Lymphocyte Count; Female; Hepatitis B - blood; Hepatitis B - enzymology; Hepatitis B virus; HIV Infections - blood; HIV Infections - diagnosis; HIV Infections - enzymology; HIV Seropositivity - blood; HIV Seropositivity - enzymology; Human immunodeficiency virus; Human viral diseases; Humans; Infectious diseases; Investigative techniques, diagnostic techniques (general aspects); Isoenzymes; Isoenzymes - blood; Male; Medical sciences; Metabolic diseases; Middle Aged; Other metabolic disorders; Purines and pyrimidines (gout, hyperuricemia...); Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Adenosine deaminase; Human immunodeficiency virus; Hepatitis B virus; Isoenzymes; Isozyme; Orthohepadnavirus; Enzymopathy; Hepadnaviridae; Clinical biology; Serum; Immunopathology; Enzyme; Retroviridae; Metabolic diseases; Adenosine deaminase deficiency; AIDS; Immune deficiency; Lentivirus; Biological activity; Genetic disease; Infection; Virus; Viral disease; Hydrolases; Purine
• ISSN: 0009-9120; 1873-2933; 1873-2933
• DOI: 10.1016/j.clinbiochem.2011.05.020

To determine adenosine deaminase (ADA) activity as a possible diagnostic marker in HIV and HIV–HBV co-infected patients. Blood samples were collected from 72 healthy, 33 HIV positive and 30 HIV–HBV co-infected subjects. Blood CD4+ cell count was recorded and serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total ADA, and ADA1 and ADA2 isoenzyme activities were determined. Serum ALT, AST, total ADA and ADA2 isoenzyme activities were significantly higher in HIV positive and HIV–HBV co-infected groups compare to the control ( p < 0.05), whereas serum ALP showed no differences between groups. CD4+ cell counts markedly decreased in all patients and showed a significant inverse correlation with ADA activities (R 2 = 0.589, p < 0.001). Serum ADA was significantly increased in HIV and HIV–HBV co-infections. Therefore, because of its low cost and simplicity to perform, ADA activity might be considered as a useful diagnostic tool among the other markers in these diseases.