Reversal Effect of BM-cyclin 1 on Multidrug Resistance by Down-regulating MRP2 in BALB/C Nude Mice Bearing C-A120 Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 33; no. 6; pp. 840 - 844
• Main Author: 王林 李肖芸 江高峰 梁继珍 孙嫣 刘炜
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.12.2013; Department of Oncology, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou Red Cross Hospital,Guangzhou 510220, China%Guangdong Provincial Key Laboratory of Liver Disease Research, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China%School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan 430065, China%Department of Gastroenterology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China
• Subjects: Animals; Antiprotozoal Agents - pharmacology; Cell Line, Tumor; Diterpenes - pharmacology; Down-Regulation; Doxorubicin - pharmacology; Drug Resistance, Multiple - drug effects; Humans; Medicine; Medicine & Public Health; Mice; Mice, Nude; Minocycline - pharmacology; Multidrug Resistance-Associated Proteins - genetics; Multidrug Resistance-Associated Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; RT-PCR; Xenograft Model Antitumor Assays; 多药耐药; 细胞周期蛋白; 耐药性; 聚合酶链反应; 裸鼠; 轴承; 逆转; multidrug resistance; BM-cyclin 1; MRP2; in vivo
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-013-1208-6

Our previous study demonstrated that BM-cyclin 1, a traditional anti-mycoplasma drug, could effectively reverse the multidrug resistance （MDR） of C-A120 cells. The present study aims to explore the reversal effect of BM-cyclin 1 on MDR and its mechanisms in BALB/C nude mice bearing C-A120 cells. Irnmunoblotting analysis and reverse transcription-polymerase chain reaction （RT-PCR） were used to study the change in multidrug resistance-associated protein 2 （MRP2） induced by BM-cyclin 1. We found that the expression levels of MRP2 protein and mRNA in C-A120 cells treated with BM-cyclin 1 were reduced significantly. Chemical colorimetry revealed no significant change in the level of glutathione （GSH）. In the xenografl model, the inhibitory rate of C-A120 cells growth in BM-cyclin 1 plus adriamycin （ADM） group was 52%, which was significantly higher than in control group （P〈0.01）. The immunoblotting and RT-PCR results conclusively demonstrated that BM-cycin 1 could significantly reduce the expression of MRP2 in transplanted tumor. In conclusion, BM-cyclin 1 could effectively reverse the MDR of C-A 120 cells in vivo by suppressing the expression of MRP2.