Exploring the role of long noncoding RNAs in immune regulation and pediatric asthma pathogenesis
Pediatric asthma, a growing chronic condition, faces diagnostic and treatment challenges in the post-COVID era. This study uses bioinformatics to explore lncRNAs' roles in pediatric asthma, aiming to improve diagnosis and treatment. To identify differentially expressed lncRNAs, the study will u...
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| Published in | Medicine (Baltimore) Vol. 104; no. 35; p. e44077 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Hagerstown, MD
Lippincott Williams & Wilkins
29.08.2025
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1536-5964 0025-7974 1536-5964 |
| DOI | 10.1097/MD.0000000000044077 |
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| Summary: | Pediatric asthma, a growing chronic condition, faces diagnostic and treatment challenges in the post-COVID era. This study uses bioinformatics to explore lncRNAs' roles in pediatric asthma, aiming to improve diagnosis and treatment. To identify differentially expressed lncRNAs, the study will utilize a 2-pronged approach: obtaining gene expression datasets from the gene expression omnibus and recruiting patients for gene sequencing. The intersection of these 2 datasets will be analyzed to pinpoint lncRNAs that exhibit differential expression. The CIBERSORT approach was employed to estimate the composition of immune-infiltrating cells within the microenvironment of each patient in the gene expression omnibus dataset. Ultimately, real-time quantitative PCR was employed to confirm the variation in lncRNA in peripheral blood. In pediatric asthma patients, MIR22HG is significantly underexpressed compared to healthy subjects. Concurrently, there is a notable increase in naive CD4 T cells in the microenvironment during asthma, along with a negative correlation between MIR22HG and naive CD4 T cells.. In summary, MIR22HG might influence the onset and progression of pediatric asthma, which could serve as a diagnostic marker for pediatric asthma and offer valuable insights for asthma diagnosis. |
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| Bibliography: | This work was supported by Medical Health Science and Technology Project Zhejiang Provincial Health Commission (Grant No. 2023KY337), Zhejiang Provincial Basic Public Welfare Research Program (LQ21H160040), There are no conflicts of interest to declare. This research was approved by the Ethics Review Board of Jiaxing Maternity and Children Health Care Hospital. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study. Participants under the age of 16 have obtained informed consent from their parents or legal guardians. The authors have no conflicts of interest to disclose. The datasets generated during and/or analyzed during the current study are publicly available. Supplemental Digital Content is available for this article. How to cite this article: Ling K, Zhang S, Jin L, Wang J. Exploring the role of long noncoding RNAs in immune regulation and pediatric asthma pathogenesis. Medicine 2025;104:35(e44077). Jiaxing Maternity and Children Health Care Hospital sequencing data in Supplemental Table 1, Supplemental Digital Content, https://links.lww.com/MD/P751. *Correspondence: Jianguo Wang, Central Laboratory, Jiaxing Women & Children's Hospital, Wenzhou Medical University, Jiaxing, Zhejiang 314000, China (e-mail: srsywjg@163.com). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1536-5964 0025-7974 1536-5964 |
| DOI: | 10.1097/MD.0000000000044077 |