Cultivable microbiome and its resistance to antimicrobials isolated from Zaprionus indianus

• Published in: Environmental monitoring and assessment Vol. 190; no. 10; p. 616
• Main Authors: da Cruz, Graziele Aparecida Fernandes, de Jesus Pires, Débora, Bataus, Luiz Artur Mendes, Silva, Rassan Dyego Romão, Barbosa, Mônica Santiago, Gomes, Raylane Pereira, Braga, Carla Afonso Silva Bitencourt, Carneiro, Lilian Carla
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.10.2018; Springer Nature B.V
• Subjects: Animals; Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; Antibiotic resistance; Antibiotics; Antimicrobial agents; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Collections; Drosophilidae - physiology; Drug resistance; Dry season; Earth and Environmental Science; Ecology; Ecosystems; Ecotoxicology; Enterobacteriaceae; Environment; Environmental factors; Environmental Management; Environmental Monitoring; Fluconazole; fungal growth; Fungi; Humans; Humidity; Identification; Insects; Integument; Ketoconazole; microbiome; Microbiomes; Microbiota - drug effects; Microorganisms; Monitoring/Environmental Analysis; parks; Parks & recreation areas; Pathogens; Plasmids; Seasons; Statistical analysis; temperature; Trichophyton; Variance analysis; Vectors; Zaprionus indianus; Resistance; Bacteria; Environmental; Microorganism; Biodiversity; Vector
• ISSN: 0167-6369; 1573-2959; 1573-2959
• DOI: 10.1007/s10661-018-6974-y

The objective of this study was to identify Z. indianus in PEJC and PESCAN. Listed are the following methods: to isolate bacteria from the integument of the Z. indianus species collected and to check the resistance of microorganisms to antibiotics. Collections of Z. indianus were performed in four seasons of the two parks. The results obtained suggest that the low amount of Z. indianus collected can be justified by environmental factors such as high average temperature and low average humidity. It is noted that there is a predominance of bacteria of the Enterobacteriaceae family found in both the PEJC and the PESCAN. The antibiogram performed for the isolated PEJC bacteria shows statistical significance when comparing the edge and inside values of the park. Studies with fungi were also carried out, and it was evidenced that Trichophyton spp. was the genus that most inhabited the two environments studied. The ability of fluconazole and ketoconazole to inhibit fungal growth was also investigated, and considering the concentration tested may suggest that they have good action spectra. Plasmid profile data show that 60% of antibiotic-resistant bacteria have plasmids. The values found show that Z. indianus can act as vectors of microorganisms that affect the healthy animals and humans and that these organisms may be influenced by seasons.