Pseudomonas pharmafabricae sp. nov., Isolated From Pharmaceutical Wastewater
A Gram-stain-negative, aerobic, rod-shaped bacterial strain, designated ZYSR67-Z T , was isolated from a pharmaceutical wastewater sample collected from a chemical factory in Zhejiang, China. The strain was motile by a single polar flagellum and grew at 4–42 °C (optimum, 35 °C), pH 5.0–9.0 (optimum,...
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Published in | Current microbiology Vol. 75; no. 9; pp. 1119 - 1125 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.09.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
ISSN | 0343-8651 1432-0991 1432-0991 |
DOI | 10.1007/s00284-018-1495-z |
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Summary: | A Gram-stain-negative, aerobic, rod-shaped bacterial strain, designated ZYSR67-Z
T
, was isolated from a pharmaceutical wastewater sample collected from a chemical factory in Zhejiang, China. The strain was motile by a single polar flagellum and grew at 4–42 °C (optimum, 35 °C), pH 5.0–9.0 (optimum, 6.0) and 0–5.0% (w/v) NaCl (optimum, 2.0%). Based on multilocus sequence analysis using 16S rRNA,
gyrB, rpoB
and
rpoD
, the strain ZYSR67-Z
T
formed a distinct phylogenetic group in the genus
Pseudomonas
. The average nucleotide identity values between strain ZYSR67-Z
T
and the closely related 10 type strains of the
Pseudomonas
species were 75.8–78.6%. The in silico DNA–DNA hybridization values indicated that strain ZYSR67-Z
T
and the type strains of the
Pseudomonas
shared 21.4–23.1% DNA relatedness. The predominant isoprenoid quinone system was ubiquinone-9 while ubiquinone-8 was present in trace amounts. The major fatty acids (> 10%) identified were C
12:0
, C
16:0
, C
18:1
ω
7
c
and summed features 3 (C
16:1
ω
7
c
and/or iso-C
15:0
2OH). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 62.6 mol%. On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain ZYSR67-Z
T
was proposed to represent a novel species of the genus
Pseudomonas
, named
Pseudomonas pharmafabricae
sp. nov.. The type strain is ZYSR67-Z
T
(= CGMCC 1.15498
T
= JCM 31306
T
). |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
ISSN: | 0343-8651 1432-0991 1432-0991 |
DOI: | 10.1007/s00284-018-1495-z |