Pseudomonas pharmafabricae sp. nov., Isolated From Pharmaceutical Wastewater

A Gram-stain-negative, aerobic, rod-shaped bacterial strain, designated ZYSR67-Z T , was isolated from a pharmaceutical wastewater sample collected from a chemical factory in Zhejiang, China. The strain was motile by a single polar flagellum and grew at 4–42 °C (optimum, 35 °C), pH 5.0–9.0 (optimum,...

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Published inCurrent microbiology Vol. 75; no. 9; pp. 1119 - 1125
Main Authors Yu, Xiao-Yun, Zhai, Jing-Yu, Wu, Chen, Zhang, Chong-Ya, Shi, Jie-Ying, Ding, Lin-Xian, Wu, Min
Format Journal Article
LanguageEnglish
Published New York Springer US 01.09.2018
Springer Nature B.V
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ISSN0343-8651
1432-0991
1432-0991
DOI10.1007/s00284-018-1495-z

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Summary:A Gram-stain-negative, aerobic, rod-shaped bacterial strain, designated ZYSR67-Z T , was isolated from a pharmaceutical wastewater sample collected from a chemical factory in Zhejiang, China. The strain was motile by a single polar flagellum and grew at 4–42 °C (optimum, 35 °C), pH 5.0–9.0 (optimum, 6.0) and 0–5.0% (w/v) NaCl (optimum, 2.0%). Based on multilocus sequence analysis using 16S rRNA, gyrB, rpoB and rpoD , the strain ZYSR67-Z T formed a distinct phylogenetic group in the genus Pseudomonas . The average nucleotide identity values between strain ZYSR67-Z T and the closely related 10 type strains of the Pseudomonas species were 75.8–78.6%. The in silico DNA–DNA hybridization values indicated that strain ZYSR67-Z T and the type strains of the Pseudomonas shared 21.4–23.1% DNA relatedness. The predominant isoprenoid quinone system was ubiquinone-9 while ubiquinone-8 was present in trace amounts. The major fatty acids (> 10%) identified were C 12:0 , C 16:0 , C 18:1 ω 7 c and summed features 3 (C 16:1 ω 7 c and/or iso-C 15:0 2OH). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 62.6 mol%. On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain ZYSR67-Z T was proposed to represent a novel species of the genus Pseudomonas , named Pseudomonas pharmafabricae sp. nov.. The type strain is ZYSR67-Z T (= CGMCC 1.15498 T  = JCM 31306 T ).
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ISSN:0343-8651
1432-0991
1432-0991
DOI:10.1007/s00284-018-1495-z