Correlation of PDCD5 and Apoptosis in Hair Cells and Spiral Ganglion Neurons of Different Age of C57BL/6J Mice

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 32; no. 1; pp. 113 - 118
• Main Author: 王燕 褚汉启 周良强 高贺云 熊浩 陈请国 陈金 黄孝文 崔永华
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2012
• Subjects: Age; age-related; Aging; Aging - pathology; Aging - physiology; Animals; Apoptosis; Apoptosis Regulatory Proteins - metabolism; Caspase-3; cell; Cells, Cultured; cells;spiral; Cochlea; death; Female; ganglion; hair; Hair cells; Hair Cells, Auditory - cytology; Hair Cells, Auditory - physiology; hearing; Hearing loss; Immunoassays; Immunohistochemistry; loss;apoptosis;programmed; Male; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; Neoplasm Proteins - metabolism; Neurons; Polymerase chain reaction; RNA; spiral ganglion; Spiral Ganglion - cytology; Spiral Ganglion - physiology; Statistics as Topic; Western blotting; hair cells; apoptosis; spiral ganglion neurons; age-related hearing loss; programmed cell death 5
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-012-0020-z

This study examined the expression pattern of programmed cell death 5 (PDCD5) in co-chlear hair cells and spiral ganglion neurons (SGNs) and its association with age-related hearing loss in mice.Sixty C57BL/6J (C57) mice at different ages were divided into four groups (3,6,9 or 12 months).PDCD5 expression was detected by using immunohistochemistry,real-time PCR and Western blot.Morphological change of the cochleae was also evaluated by using immunoassay.The results showed that the expression of PDCD5 had a gradual increase with ageing in both protein and RNA levels in C57 mice,as well as gradually increased apoptosis of cochlear hair cells and SGNs.In addition,we also found that caspase-3 activity was enhanced and its expression was enhanced with ageing.It is implied that overexpression of PDCD5 causes the increase in caspase-3 activity and the subsequent increase of apoptosis in cochlear hair cells and SGNs,and thereby plays a role in the pathogenesis of presbycusis.Thus,PDCD5 may be a new target site for the treatment and prevention of age-related hearing loss.