Correlation between Physical Status of Human Papilloma Virus and Cervical Carcinogenesis

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 32; no. 1; pp. 97 - 102
• Main Author: 李科珍 金鑫 方勇 王常玉 龚妹 陈平波 刘佳 邓东锐 艾继辉
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2012
• Subjects: Adult; Aged; Biopsy; cancer;genotyping;physical; Carcinogenesis; Cervical cancer; Cervix; China; Female; Genotyping; human; Human papillomavirus; Human papillomavirus 16 - genetics; Human papillomavirus 16 - isolation & purification; Humans; Infection; Integration; Medicine; Medicine & Public Health; Middle Aged; papilloma; Papillomavirus Infections - diagnosis; Papillomavirus Infections - virology; Polymerase chain reaction; Precancerous Conditions - diagnosis; Precancerous Conditions - virology; Primers; Prognosis; Risk Factors; Statistics as Topic; status;integration; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - virology; virus;cervical; China; integration; physical status; human papilloma virus; cervical cancer; genotyping
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-012-0017-7

The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer,the physical status of HPV-16 in patients with cervical lesions,and the role of HPV-16 integration in cervi-cal carcinogenesis were investigated.HPV genotyping was performed by using PCR approach with the primer GP5+/GP6+ and type-specific primer on biopsy specimens taken operatively from 198 women.Multiple PCR was done to detect physical status of HPV-16 in a series of cervical liquid-based cytology samples and biopsy specimens obtained from different cervical lesions with HPV-16 infection,includ-ing 112 specimens with cervical cancer,151 specimens with CINⅠ,246 specimens with CINⅡ and 120 specimens with CINⅢ.The results showed that there were 112 cervical cancer samples (56.57% of total cervical cancer patients) with HPV-16 infection.The frequency of HPV-16 pure integration was 65.18% (73/112),56.57% (47/120),23.58% (58/246) and 7.95% (12/151) in cervical cancer,CINⅢ,CINⅡand CINⅠ patients respectively.In situ hybridization was performed on some paraffin-embedded sections of CINⅡ,CINⅢ and cervical cancer to verify the physical status of HPV-16 infection.Sig-nificant difference was observed between cervical cancer and CINⅠ,CINⅡ,CINⅢ in the frequency of HPV-16 integration (P0.01).It is suggested that HPV-16 is the most prevalent type and is associated with cervical cancer.In the case of HPV-16 infection there are close associations between the severity of cervical lesions and the frequency of HPV-16 integration.The application of testing HPV genotyping and physical status based on detection of HC-Ⅱ HPV DNA would be in favor of predicting the progno-sis of cervical precancerosis and enhancing the screening accuracy of cervical cancer.