Two birds with one stone: A universal design and application of signal-on labeled fluorescent/electrochemical dual-signal mode biosensor for the detection of tetracycline residues in tap water, milk and chicken

• Published in: Food chemistry Vol. 430; p. 136904
• Main Authors: Yang, Lanqing, Wang, Xiaomin, Zhang, Fuyuan, Yu, Ligang, Bai, Baoqing, Zhang, Jinhua, Zhang, Bo, Tian, Yu, Qin, Shu, Yang, Yukun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2024
• Subjects: Biosensor; biosensors; chickens; detection limit; Dual-signal mode; Electrochemical; electrochemistry; fluorescence; Fluorescent; food chemistry; magnetic separation; methylene blue; milk; tap water; Tetracycline; Fluorescent; Biosensor; Dual-signal mode; Electrochemical; Tetracycline
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2023.136904

•An FL/EC dual-signal mode sensing platform was constructed for the detection of TET.•The capture probe Fe3O4-Apt and dual-signal tag cDNA-MOF-MB were prepared.•The biosensing platform (Fe3O4-Apt/cDNA-MOF-MB) was constructed.•The biosensor exhibited wide linear range and good detection accuracy.•The design of dual-mode sensing based on different signaling systems can be promoted. An ingenious and universal design of fluorescent/electrochemical dual-signal mode sensing platform was constructed for the sensitive, selective and accurate detection of tetracycline (TET). Apt-functionalized nano-magnetic beads (Fe3O4-Apt) as capture probe, Apt-complementary short-chain functionalized fluorescent MOF loaded with methylene blue (MB) (cDNA-MOF-MB) as dual-signal tag were prepared. The sensing platform (Fe3O4-Apt/cDNA-MOF-MB) was formed based on the base complementary pairing of Apt and cDNA. With the help of Apt for target recognition, together with magnetic separation technology, a dual-signal mode biosensor was constructed. The dual-signal mode biosensor exhibited a wide linear concentration range from 1.00 × 10−9 g/mL to 1.00 × 10−4 g/mL with a low limit of detection (LOD) of 1.69 × 10−10 g/mL (fluorescence mode assay) and 1.15 × 10−10 g/mL (electrochemical mode assay). The proposed biosensor had been successfully applied to the determination of TET content in real samples with satisfactory recoveries (94.99–101.30%).