Effects of stirring speed ladder on the acid-promoted refolding of rice glutelin

• Published in: International journal of biological macromolecules Vol. 228; pp. 216 - 223
• Main Authors: He, Chengxin, Song, Liwen, Liu, Ziwei, Xiong, Hua, Zhao, Qiang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.02.2023
• Subjects: acidification; Aggregation; Computational fluid dynamics; glutelins; Glutens - metabolism; hydrocolloids; hydrophobicity; molecular weight; Oryza - metabolism; particle size; pH-cycle; Principal component analysis; quality control; rice; Rice glutelin; Solubility; Stirring speed; Aggregation; Computational fluid dynamics; Rice glutelin; Solubility; pH-cycle; Stirring speed; Principal component analysis
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.12.119

The effects of stirring speed (0, 250, 500, 750, 1000, 1250, and 1500 rpm) on the rice glutelin hydrocolloids (1 %, w/v) during the acidified process were investigated. As the stirring speed was increased to 750 rpm, the hydration diameter of the rice glutelin was significantly decreased, but higher stirring speeds had no significant effect on size. The highest and lowest solubility were recorded for the samples treated at 750 and 0 rpm stirring speeds, respectively. The surface hydrophobicity and molecular weight increased first and then decreased, both the minimum value was recorded at 750 rpm sample. The principal component analysis (PCA) was employed to detect patterns between changes in various properties (solubility, particle size, β-sheet content, surface hydrophobicity, and ζ-potential) and stirring treatment. To conclude, the various properties of rice glutelin refold during acidification are drastically affected by employing different stirring speeds. Choosing a suitable stirring speed is important for quality control in protein hydrocolloid production. •Stirring changes the interface properties and molecular weight of rice glutelin.•The size of rice glutelin decreases with the stirring speed increase at 0–750 rpm.•The stirring speed of 750 rpm obtained maximum solubility of rice glutelin.•The stirring speed of 750 rpm is sufficient to form a swirl.