Lactobacillus rhamnosus Strain LRH05 Intervention Ameliorated Body Weight Gain and Adipose Inflammation via Modulating the Gut Microbiota in High‐Fat Diet‐Induced Obese Mice

• Published in: Molecular nutrition & food research Vol. 66; no. 1; pp. e2100348 - n/a
• Main Authors: Chen, Yung‐Tsung, Chiou, Shiou‐Yun, Hsu, Ai‐Hua, Lin, Yu‐Chun, Lin, Jin‐Seng
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.01.2022
• Subjects: Adipogenesis; Adipose tissue; Animals; Body weight; body weight changes; Body weight gain; Butyric acid; Diet; Diet, High-Fat - adverse effects; Dosage; Fatty liver; food research; Fusobacterium; Gastrointestinal Microbiome; Gene expression; glucose; Glucose tolerance; Gut microbiota; High fat diet; Infiltration; Inflammation; Inflammatory response; Insulin-like growth factors; Intestinal microflora; intestinal microorganisms; Intolerance; Lactobacilli; Lactobacillus reuteri; Lactobacillus rhamnosus; Macrophages; Mice; Mice, Inbred C57BL; Mice, Obese; Microbiota; Obesity; Probiotics; Probiotics - pharmacology; Propionic acid; Steatosis; triacylglycerols; Triglycerides; Weight Gain; Weight reduction; white adipose tissue; macrophages; inflammation; gut microbiota; obesity; probiotics
• ISSN: 1613-4125; 1613-4133; 1613-4133
• DOI: 10.1002/mnfr.202100348

Scope This study aims to investigate the underlying mechanism of a specific probiotic strain on suppression of adipogenesis and inflammatory response in white adipose tissue (WAT) of high‐fat diet (HFD)‐fed mice. Methods and Results Eight strains are screened in vitro for candidates of potential probiotics. Lactobacillus rhamnosus LRH05 (LRH05) and Lactobacillus reuteri LR47 (LR47) are screened out with lower triglyceride expression in vitro. The mice are fed a control diet (CD), HFD, or HFD supplemented with a dose of LRH05 or LR47 at 109 CFU per mouse per day for 10 weeks (n = 8), respectively. The results demonstrate that LRH05, but not LR47, significantly reduce body weight gain and the weight of WAT, as well as improve hepatic steatosis and glucose intolerance. LRH05 regulates the Mogat1, Igf‐1, Mcp‐1, and F4/80 mRNA expression and decreases macrophage infiltration in WAT. LRH05 shows an increase in butyric and propionic acid‐producing bacteria, including Lachnoclostridium, Romboutsia, and Fusobacterium that is coincident with the increased fecal propionic acid and butyric acid levels. Conclusion LRH05 shows a strain‐specific effect on ameliorating the pro‐inflammatory process by reducing inflammatory macrophage infiltration and the expression of inflammation‐related genes in mice. Thus, LRH05 can be considered a potential probiotic strain to prevent obesity. This study aims to investigate the underlying mechanism of a Lactobacillus rhamnosus LRH05 on suppression of adipogenesis and inflammatory response in white adipose tissue (WAT) of high‐fat diet (HFD)‐fed mice. The results demonstrate that LRH05 significantly reduces body weight gain and the weight of WAT, as well as improves hepatic steatosis and glucose intolerance. Moreover, LRH05 regulates the Mogat1, Igf‐1, Mcp‐1, and F4/80 mRNA expression and decreases macrophage infiltration in WAT, as well as increases fecal propionic acid and butyric acid levels through manipulation of gut microbiota composition.