Topical application of superoxide dismutase mediated by HIV-TAT peptide attenuates UVB-induced damages in human skin

• Published in: European journal of pharmaceutics and biopharmaceutics Vol. 107; pp. 286 - 294
• Main Authors: Chen, Xiaochao, Liu, Shutao, Rao, Pingfan, Bradshaw, Jeremy, Weller, Richard
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2016
• Subjects: Administration, Topical; Blood flow; Erythema; Erythema - prevention & control; HIV-TAT peptide; Humans; Male; Microscopy, Confocal; Peptides - chemistry; Permeability; Skin - radiation effects; Spectrophotometry, Ultraviolet; Sunburn cell; Superoxide dismutase; Superoxide Dismutase - administration & dosage; Tape stripping; tat Gene Products, Human Immunodeficiency Virus - chemistry; Transdermal delivery; Ultraviolet; Ultraviolet Rays; UV; CPP; TAT; Tape stripping; SOD; Superoxide dismutase; Ultraviolet; MED; Blood flow; GFP; SC; HIV-TAT peptide; Sunburn cell; HIV; SBC; ROS; Transdermal delivery; Erythema
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/j.ejpb.2016.07.023

[Display omitted] •Topical application of superoxide dismutase can be enhanced by binding to HIV-TAT peptide.•TAT-SOD protein efficiently penetrated through the stratum corneum.•TAT-SOD protein significantly attenuated UVB-induced skin damage in man. The purpose of this study was to evaluate whether topical application of superoxide dismutase with cell penetrating peptide (HIV-TAT) could protect against skin damage induced by UVB irradiation in humans. The permeability through stratum corneum of large proteins linked to TAT peptide was firstly confirmed by confocal microscopy and tape stripping. Ten healthy volunteers with either Fitzpatrick skin type II or III were recruited in this clinical study. TAT-SOD (300units/cm2) and vehicle cream were applied on two symmetric areas of both inner upper arms 1h prior to UVB irradiation. After one hour of pretreatment, subjects received 10 incremental doses of UVB on pretreated areas. 24h later, erythema, blood flow and apoptotic cells were measured. Pretreatment with TAT-SOD 1h prior to UVB radiation promoted a mean minimal erythema dose (MED) increase of 36.6±18.4% (p=0.013<0.05. n=10) compared to vehicle control. The median blood flow values of all subjects following 2 and 3-MED of UVB were 107.8±51.0units and 239.5±88.0units respectively, which account for 26% and 25% decrease with respect to vehicle groups. These data suggest that TAT-SOD significantly suppresses UVB induced erythema formation and blood flow rise. Furthermore, pretreatment with TAT-SOD 1h prior to 2-MED of UVB irradiation reduced the apoptotic sunburn cell formation by 47.6±8.6% (p<0.0001) in all subjects. Evaluating results generated from all measurements, we conclude that topical application of TAT-SOD significantly attenuates UVB-induced skin damage in man. These biological effects of TAT-SOD are probably mediated via its free radical scavenging properties, clearly differentiating it from other physical sunscreen agents.