Sequencing and bacterial expression of a novel murine alpha interferon gene
A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cel...
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| Published in | Science China. Life sciences Vol. 40; no. 3; pp. 277 - 283 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
China
Springer Nature B.V
01.06.1997
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-7305 1006-9305 1869-1889 1862-2798 |
| DOI | 10.1007/BF02879088 |
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| Abstract | A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mlFN-αB has been accepted by GENEBANK. |
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| AbstractList | A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the P^sub L^ promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK.[PUBLICATION ABSTRACT] A murine new alpha interferon gene (mIFN- alpha B) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the P sub(L) promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN- alpha B has been accepted by GENEBANK. A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mlFN-αB has been accepted by GENEBANK. A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK. A murine new alpha interferon gene (mIFN-alphaB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the P(L) promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-alphaB has been accepted by GENEBANK.A murine new alpha interferon gene (mIFN-alphaB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the P(L) promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-alphaB has been accepted by GENEBANK. A murine new alpha interferon gene (mIFN-alphaB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the P(L) promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-alphaB has been accepted by GENEBANK. |
| Author | 王焱 王征宇 周鸣南 蔡菊娥 孙兰英 刘新垣 B.L.Daugherty S.Pestka |
| AuthorAffiliation | Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai 200031 China Department of Molecular Genetics and Microbiology UMDNJ-Robert Wood Johnson Medical School New Jersey USA USA |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/18726328$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/S0006-291X(86)80571-X 10.1093/nar/13.3.791 10.1089/jir.1984.4.635 |
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| Notes | WANG Yan I WANG Zhengyu , ZHOU MingnanCAIJu’e SUN Lanying LIU Xinyuan(Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China)B.L. Daugherty and S. Pestka(Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, New Jersey, USA) 11-5841/Q ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Feature-1 ObjectType-Article-2 content type line 23 |
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| References | M. Dion (BF02879088_CR3) 1986; 138 D. Bruce (BF02879088_CR1) 1984; 4 E. E. Zwarthoff (BF02879088_CR2) 1985; 13 |
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| Snippet | A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence... A murine new alpha interferon gene (mIFN-alphaB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its... A murine new alpha interferon gene (mIFN- alpha B) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its... |
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| SubjectTerms | alpha Antiviral activity expression Genes Interferon method murine Nucleotide sequence primer-based prokaryotic sequencing |
| Title | Sequencing and bacterial expression of a novel murine alpha interferon gene |
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